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10.7554/eLife.16616 http://scihub22266oqcxt.onion/10.7554/eLife.16616 C5026471!5026471!27490484     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       eLife 2016 ; 5 (ä): ä Nephropedia Template TP {{tp|p=27490484|t=2016. The selectivity of the Na+/K+-pump is controlled by binding site protonation and self-correcting occlusion |pdf=|usr=}}{{27490484}} gab.com Text The selectivity of the Na+/K+-pump is controlled by binding site protonation and self-correcting occlusion JO: eLife http://www.kidney.de/pget.php?&tw=1&pmid=27490484 #FOAvip The Na+/K+-pump maintains the physiological K+ and Na+ electrochemical gradients across the cell membrane. It operates via an 'alternating-access' mechanism, making iterative transitions between inward-facing (E1) and outward-facing (E2) conformations. Although the general features of the transport cycle are known, the detailed physicochemical factors governing the binding site selectivity remain mysterious. Free energy molecular dynamics simulations show that the ion binding sites switch their binding specificity in E1 and E2. This is accompanied by small structural arrangements and changes in protonation states of the coordinating residues. Additional computations on structural models of the intermediate states along the conformational transition pathway reveal that the free energy barrier toward the occlusion step is considerably increased when the wrong type of ion is loaded into the binding pocket, prohibiting the pump cycle from proceeding forward. This self-correcting mechanism strengthens the overall transport selectivity and protects the stoichiometry of the pump cycle.DOI:http://dx.doi.org/10.7554/eLife.16616.001 Twit Text FOAVip The selectivity of the Na+/K+-pump is controlled by binding site protonation and self-correcting occlusion ????eLife http://www.kidney.de/pget.php?&tw=1&pmid=27490484 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27490484 #FOAvip English Wikipedia <ref>{{Cite pmid|27490484}}</ref> The selectivity of the Na+/K+-pump is controlled by binding site protonation and self-correcting occlusion #MMPMID27490484 Rui H; Artigas P; Roux B eLife 2016[]; 5 (ä): ä PMID27490484show ga The Na+/K+-pump maintains the physiological K+ and Na+ electrochemical gradients across the cell membrane. It operates via an 'alternating-access' mechanism, making iterative transitions between inward-facing (E1) and outward-facing (E2) conformations. Although the general features of the transport cycle are known, the detailed physicochemical factors governing the binding site selectivity remain mysterious. Free energy molecular dynamics simulations show that the ion binding sites switch their binding specificity in E1 and E2. This is accompanied by small structural arrangements and changes in protonation states of the coordinating residues. Additional computations on structural models of the intermediate states along the conformational transition pathway reveal that the free energy barrier toward the occlusion step is considerably increased when the wrong type of ion is loaded into the binding pocket, prohibiting the pump cycle from proceeding forward. This self-correcting mechanism strengthens the overall transport selectivity and protects the stoichiometry of the pump cycle.DOI:http://dx.doi.org/10.7554/eLife.16616.001 äThe selectivity of the Na+/K+-pump is controlled by binding site proto(epmc).pdf DeepDyve Pubget Overpricing