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2016 ; 19
(5
): 445-9
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Vitality of Enterococcus faecalis inside dentinal tubules after five root canal
disinfection methods
#MMPMID27656064
Vatkar NA
; Hegde V
; Sathe S
J Conserv Dent
2016[Sep]; 19
(5
): 445-9
PMID27656064
show ga
AIM: To compare the vitality of Enterococcus faecalis within dentinal tubules
after subjected to five root canal disinfection methods. MATERIALS AND METHODS:
Dentin blocks (n = 60) were colonized with E. faecalis. After 4 weeks of
incubation, the dentin blocks were divided into one control and five test groups
(n = 10 each). The root canals of test groups were subjected to one of the
disinfection methods, namely, normal saline (NS), sodium hypochlorite (NaOCl),
chlorhexidine digluconate (CHX), neodymium-doped yttrium aluminum garnet (Nd:
YAG) laser, and diode laser. The effect of disinfection methods was assessed by
LIVE/DEAD BacLight stain under the confocal laser scanning microscopy to
determine the "zone of dead bacteria" (ZDB). Mean values were calculated for ZDB
and the difference between groups was established. RESULTS: Penetration of E.
faecalis was seen to a depth of >1000 ?m. Viable bacteria were detected with NS
irrigation. NaOCl and CHX showed partial ZDB. When the root canals were
disinfected with Nd: YAG and diode lasers, no viable bacteria were found.
CONCLUSION: E. faecalis has the ability to colonize inside dentinal tubules to a
depth of >1000 ?m. In contrast to conventional irrigants, both Nd: YAG and diode
lasers were effective in eliminating the vitality of E. faecalis. NS, NaOCl, and
CHX showed viable bacteria remaining in dentinal tubules.