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10.1074/jbc.M116.726471 http://scihub22266oqcxt.onion/10.1074/jbc.M116.726471 C5025673!5025673!27489102     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2016 ; 291 (38): 19835-47 Nephropedia Template TP {{tp|p=27489102|t=2016. Activation of the Human Epithelial Sodium Channel (ENaC) by Bile Acids Involves the Degenerin Site* |pdf=|usr=}}{{27489102}} gab.com Text Activation of the Human Epithelial Sodium Channel (ENaC) by Bile Acids Involves the Degenerin Site* JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27489102 #FOAvip The epithelial sodium channel (ENaC) is a member of the ENaC/degenerin ion channel family, which also includes the bile acid-sensitive ion channel (BASIC). So far little is known about the effects of bile acids on ENaC function. ENaC is probably a heterotrimer consisting of three well characterized subunits (???). In humans, but not in mice and rats, an additional ?-subunit exists. The aim of this study was to investigate the effects of chenodeoxycholic, cholic, and deoxycholic acid in unconjugated (CDCA, CA, and DCA) and tauro-conjugated (t-CDCA, t-CA, t-DCA) form on human ENaC in its ???- and ???-configuration. We demonstrated that tauro-conjugated bile acids significantly stimulate ENaC in the ???- and in the ???-configuration. In contrast, non-conjugated bile acids have a robust stimulatory effect only on ???ENaC. Bile acids stimulate ENaC-mediated currents by increasing the open probability of active channels without recruiting additional near-silent channels known to be activated by proteases. Stimulation of ENaC activity by bile acids is accompanied by a significant reduction of the single-channel current amplitude, indicating an interaction of bile acids with a region close to the channel pore. Analysis of the known ASIC1 (acid-sensing ion channel) crystal structure suggested that bile acids may bind to the pore region at the degenerin site of ENaC. Substitution of a single amino acid residue within the degenerin region of ?ENaC (N521C or N521A) significantly reduced the stimulatory effect of bile acids on ENaC, suggesting that this site is critical for the functional interaction of bile acids with the channel. Twit Text FOAVip Activation of the Human Epithelial Sodium Channel (ENaC) by Bile Acids Involves the Degenerin Site* ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=27489102 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27489102 #FOAvip English Wikipedia <ref>{{Cite pmid|27489102}}</ref> Activation of the Human Epithelial Sodium Channel (ENaC) by Bile Acids Involves the Degenerin Site* #MMPMID27489102 Ilyaskin AV; Diakov A; Korbmacher C; Haerteis S J Biol Chem 2016[Sep]; 291 (38): 19835-47 PMID27489102show ga The epithelial sodium channel (ENaC) is a member of the ENaC/degenerin ion channel family, which also includes the bile acid-sensitive ion channel (BASIC). So far little is known about the effects of bile acids on ENaC function. ENaC is probably a heterotrimer consisting of three well characterized subunits (???). In humans, but not in mice and rats, an additional ?-subunit exists. The aim of this study was to investigate the effects of chenodeoxycholic, cholic, and deoxycholic acid in unconjugated (CDCA, CA, and DCA) and tauro-conjugated (t-CDCA, t-CA, t-DCA) form on human ENaC in its ???- and ???-configuration. We demonstrated that tauro-conjugated bile acids significantly stimulate ENaC in the ???- and in the ???-configuration. In contrast, non-conjugated bile acids have a robust stimulatory effect only on ???ENaC. Bile acids stimulate ENaC-mediated currents by increasing the open probability of active channels without recruiting additional near-silent channels known to be activated by proteases. Stimulation of ENaC activity by bile acids is accompanied by a significant reduction of the single-channel current amplitude, indicating an interaction of bile acids with a region close to the channel pore. Analysis of the known ASIC1 (acid-sensing ion channel) crystal structure suggested that bile acids may bind to the pore region at the degenerin site of ENaC. Substitution of a single amino acid residue within the degenerin region of ?ENaC (N521C or N521A) significantly reduced the stimulatory effect of bile acids on ENaC, suggesting that this site is critical for the functional interaction of bile acids with the channel. äActivation of the Human Epithelial Sodium Channel (ENaC) by Bile Acids(epmc).pdf DeepDyve Pubget Overpricing