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10.1186/s13059-016-1038-5 http://scihub22266oqcxt.onion/10.1186/s13059-016-1038-5 C5025552!5025552 !27634179     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27634179 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Genome+Biol 2016 ; 17 (1 ): 186 Nephropedia Template TP {{tp|p=27634179 |t=2016. An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease |pdf=|usr=}}{{27634179 }} gab.com Text An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=27634179 #FOAvip Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3' flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3' flap structure formed between the target and the guide DNA (gDNA) and cut the target through its Fn1 dimerization. Our results show that the SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and endogenous genes in zebrafish embryonic genome. Twit Text FOAVip An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=27634179 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27634179 #FOAvip English Wikipedia <ref>{{Cite pmid|27634179 }}</ref> An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease #MMPMID27634179 Xu S ; Cao S ; Zou B ; Yue Y ; Gu C ; Chen X ; Wang P ; Dong X ; Xiang Z ; Li K ; Zhu M ; Zhao Q ; Zhou G Genome Biol 2016[Sep]; 17 (1 ): 186 PMID27634179 show ga Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3' flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3' flap structure formed between the target and the guide DNA (gDNA) and cut the target through its Fn1 dimerization. Our results show that the SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and endogenous genes in zebrafish embryonic genome. |*Gene Editing [MESH] |Animals [MESH] |Base Sequence [MESH] |DNA, Single-Stranded/genetics/metabolism [MESH] |DNA/chemistry/*genetics/metabolism [MESH] |Deoxyribonucleases, Type II Site-Specific/chemistry/genetics/metabolism [MESH] |Endodeoxyribonucleases/chemistry/genetics/*metabolism [MESH] |Flap Endonucleases/chemistry/genetics/metabolism [MESH] |Gene Targeting/methods [MESH] |Models, Biological [MESH] |Mutation [MESH] |Sequence Analysis, DNA [MESH] |Substrate Specificity [MESH]An alternative novel tool for DNA editing without target sequence limi(epmc).pdf DeepDyve Pubget Overpricing