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10.1073/pnas.1602202113

http://scihub22266oqcxt.onion/10.1073/pnas.1602202113
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C5024634!5024634!27535934
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suck abstract from ncbi


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pmid27535934      Proc+Natl+Acad+Sci+U+S+A 2016 ; 113 (35): 9716-21
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  • Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast #MMPMID27535934
  • Dong B; Almassalha LM; Stypula-Cyrus Y; Urban BE; Chandler JE; Nguyen TQ; Sun C; Zhang HF; Backman V
  • Proc Natl Acad Sci U S A 2016[Aug]; 113 (35): 9716-21 PMID27535934show ga
  • Fluorescence photoswitching of native, unmodified deoxyribonucleic acid (DNA) using visible light facilitates the label-free nanoscale imaging of chromatin structures based on the principle of single-molecule photon localization microscopy (PLM). With a demonstrated sub?20-nm resolution, DNA-PLM provides an ideal technique to visualize the spatial organization of single or groups of nucleosomes and quantitatively estimate the nucleosome occupancy level of DNA in unstained chromosomes and nuclei. This study paves a way for revealing nanoscopic features of chromatin without the need for exogenous labels and could substantially expand our understanding of the structure?function relationship of chromatin.
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