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10.1073/pnas.1602202113 http://scihub22266oqcxt.onion/10.1073/pnas.1602202113 C5024634!5024634 !27535934     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27535934 &cmd=llinks): Failed to open stream: HTTP request failed! 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Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast |pdf=|usr=}}{{27535934 }} gab.com Text Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27535934 #FOAvip Visualizing the nanoscale intracellular structures formed by nucleic acids, such as chromatin, in nonperturbed, structurally and dynamically complex cellular systems, will help expand our understanding of biological processes and open the next frontier for biological discovery. Traditional superresolution techniques to visualize subdiffractional macromolecular structures formed by nucleic acids require exogenous labels that may perturb cell function and change the very molecular processes they intend to study, especially at the extremely high label densities required for superresolution. However, despite tremendous interest and demonstrated need, label-free optical superresolution imaging of nucleotide topology under native nonperturbing conditions has never been possible. Here we investigate a photoswitching process of native nucleotides and present the demonstration of subdiffraction-resolution imaging of cellular structures using intrinsic contrast from unmodified DNA based on the principle of single-molecule photon localization microscopy (PLM). Using DNA-PLM, we achieved nanoscopic imaging of interphase nuclei and mitotic chromosomes, allowing a quantitative analysis of the DNA occupancy level and a subdiffractional analysis of the chromosomal organization. This study may pave a new way for label-free superresolution nanoscopic imaging of macromolecular structures with nucleotide topologies and could contribute to the development of new DNA-based contrast agents for superresolution imaging. Twit Text FOAVip Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27535934 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27535934 #FOAvip English Wikipedia <ref>{{Cite pmid|27535934 }}</ref> Superresolution intrinsic fluorescence imaging of chromatin utilizing native, unmodified nucleic acids for contrast #MMPMID27535934 Dong B ; Almassalha LM ; Stypula-Cyrus Y ; Urban BE ; Chandler JE ; Nguyen TQ ; Sun C ; Zhang HF ; Backman V Proc Natl Acad Sci U S A 2016[Aug]; 113 (35 ): 9716-21 PMID27535934 show ga Visualizing the nanoscale intracellular structures formed by nucleic acids, such as chromatin, in nonperturbed, structurally and dynamically complex cellular systems, will help expand our understanding of biological processes and open the next frontier for biological discovery. Traditional superresolution techniques to visualize subdiffractional macromolecular structures formed by nucleic acids require exogenous labels that may perturb cell function and change the very molecular processes they intend to study, especially at the extremely high label densities required for superresolution. However, despite tremendous interest and demonstrated need, label-free optical superresolution imaging of nucleotide topology under native nonperturbing conditions has never been possible. Here we investigate a photoswitching process of native nucleotides and present the demonstration of subdiffraction-resolution imaging of cellular structures using intrinsic contrast from unmodified DNA based on the principle of single-molecule photon localization microscopy (PLM). Using DNA-PLM, we achieved nanoscopic imaging of interphase nuclei and mitotic chromosomes, allowing a quantitative analysis of the DNA occupancy level and a subdiffractional analysis of the chromosomal organization. This study may pave a new way for label-free superresolution nanoscopic imaging of macromolecular structures with nucleotide topologies and could contribute to the development of new DNA-based contrast agents for superresolution imaging. |Cell Nucleus/*ultrastructure [MESH] |Chromatin/*ultrastructure [MESH] |DNA/chemistry [MESH] |HeLa Cells [MESH] |Humans [MESH] |Interphase [MESH] |Microscopy, Fluorescence/instrumentation/*methods [MESH] |Mitosis [MESH] |Nucleotides/chemistry [MESH] |Optical Imaging/instrumentation/*methods [MESH]Superresolution intrinsic fluorescence imaging of chromatin utilizing (epmc).pdf DeepDyve Pubget Overpricing