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10.1186/s12917-016-0808-3 http://scihub22266oqcxt.onion/10.1186/s12917-016-0808-3 C5024444!5024444!27630098     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Vet+Res 2016 ; 12 (ä): ä Nephropedia Template TP {{tp|p=27630098|t=2016. Techniques for thoracic duct cannulation without thoracotomy in piglets |pdf=|usr=}}{{27630098}} gab.com Text Techniques for thoracic duct cannulation without thoracotomy in piglets JO: BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=27630098 #FOAvip Background: Pigs are the natural hosts of many zoonotic pathogens such as influenza viruses and Staphylococcus aureus and thus have advantages over non-natural hosts when studying these zoonotic diseases. In addition, pulmonary infections are a key issue in the pig industry, for example: porcine reproductive and respiratory syndrome virus infection. Exploration of the pathogenesis of swine pulmonary infections, in particular at the onset of disease, will provide valuable information for the development of vaccines against these diseases. Therefore, there is need to develop a methodology that allows for in vivo sampling of efferent pulmonary lymph with minimum damage to the target tissues for studying the pathogenesis of swine pulmonary infections. Results: We introduce the surgical procedures for cannulating the thoracic duct at its point of entry into the external jugular vein cranial to the first rib on the left in pigs. Using this methodology, we monitored the amounts of triglyceride and cholesterol in the lymph collected from the thoracic duct following 30 h fasting and at multiple time points after meals. It was found that the levels of triglyceride rather than cholesterol corresponded to the milky appearance of the lymph samples. Conclusions: Our techniques provide a strategy for collecting lymph including pulmonary lymph from the thoracic duct without thoracotomy. A pig model for collecting in vivo, in situ efferent lymph draining the lower respiratory tract and its local lymph nodes in real-time with minimal tissue damage to the target tissues opens a new door for studying disease processes in pulmonary infections. Techniques described here are the key to this door. Electronic supplementary material: The online version of this article (doi:10.1186/s12917-016-0808-3) contains supplementary material, which is available to authorized users. Twit Text FOAVip Techniques for thoracic duct cannulation without thoracotomy in piglets ????BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=27630098 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27630098 #FOAvip English Wikipedia <ref>{{Cite pmid|27630098}}</ref> Techniques for thoracic duct cannulation without thoracotomy in piglets #MMPMID27630098 Yen HH; Davies HMS BMC Vet Res 2016[]; 12 (ä): ä PMID27630098show ga Background: Pigs are the natural hosts of many zoonotic pathogens such as influenza viruses and Staphylococcus aureus and thus have advantages over non-natural hosts when studying these zoonotic diseases. In addition, pulmonary infections are a key issue in the pig industry, for example: porcine reproductive and respiratory syndrome virus infection. Exploration of the pathogenesis of swine pulmonary infections, in particular at the onset of disease, will provide valuable information for the development of vaccines against these diseases. Therefore, there is need to develop a methodology that allows for in vivo sampling of efferent pulmonary lymph with minimum damage to the target tissues for studying the pathogenesis of swine pulmonary infections. Results: We introduce the surgical procedures for cannulating the thoracic duct at its point of entry into the external jugular vein cranial to the first rib on the left in pigs. Using this methodology, we monitored the amounts of triglyceride and cholesterol in the lymph collected from the thoracic duct following 30 h fasting and at multiple time points after meals. It was found that the levels of triglyceride rather than cholesterol corresponded to the milky appearance of the lymph samples. Conclusions: Our techniques provide a strategy for collecting lymph including pulmonary lymph from the thoracic duct without thoracotomy. A pig model for collecting in vivo, in situ efferent lymph draining the lower respiratory tract and its local lymph nodes in real-time with minimal tissue damage to the target tissues opens a new door for studying disease processes in pulmonary infections. Techniques described here are the key to this door. Electronic supplementary material: The online version of this article (doi:10.1186/s12917-016-0808-3) contains supplementary material, which is available to authorized users. äTechniques for thoracic duct cannulation without thoracotomy in piglet(epmc).pdf DeepDyve Pubget Overpricing