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10.1038/mtna.2016.63 http://scihub22266oqcxt.onion/10.1038/mtna.2016.63 C5023407!5023407!27574785     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Ther+Nucleic+Acids 2016 ; 5 (8): e359- Nephropedia Template TP {{tp|p=27574785|t=2016. Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery |pdf=|usr=}}{{27574785}} gab.com Text Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery JO: Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=27574785 #FOAvip Hydrodynamic gene delivery is a common method for gene transfer to the liver of small animals, and its clinical applicability in large animals has been demonstrated. Previous studies focused on functional analyses of therapeutic genes in animals with normal livers and little, however, is known regarding its effectiveness and safety in animals with liver fibrosis. Therefore, this study aimed to examine the effects of liver fibrosis on hydrodynamic gene delivery efficiency using a rat liver fibrosis model. We demonstrated for the first time, using pCMV-Luc plasmid, that this procedure is safe and that the amount of fibrotic tissue in the liver decreases gene delivery efficiency, resulting in decrease in luciferase activity depending on the volume of fibrotic tissue in the liver and the number of hepatocytes that are immunohistochemically stained positive for transgene product. We further demonstrate that antifibrotic gene therapy with matrix metalloproteinase-13 gene reduces liver fibrosis and improves efficiency of hydrodynamic gene delivery. These results demonstrate the negative effects of fibrotic tissue on hydrodynamic gene delivery and its recovery by appropriate antifibrotic therapy. Twit Text FOAVip Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery ????Mol Ther Nucleic Acids http://www.kidney.de/pget.php?&tw=1&pmid=27574785 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27574785 #FOAvip English Wikipedia <ref>{{Cite pmid|27574785}}</ref> Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery #MMPMID27574785 Kobayashi Y; Kamimura K; Abe H; Yokoo T; Ogawa K; Shinagawa-Kobayashi Y; Goto R; Inoue R; Ohtsuka M; Miura H; Kanefuji T; Suda T; Tsuchida M; Aoyagi Y; Zhang G; Liu D; Terai S Mol Ther Nucleic Acids 2016[Aug]; 5 (8): e359- PMID27574785show ga Hydrodynamic gene delivery is a common method for gene transfer to the liver of small animals, and its clinical applicability in large animals has been demonstrated. Previous studies focused on functional analyses of therapeutic genes in animals with normal livers and little, however, is known regarding its effectiveness and safety in animals with liver fibrosis. Therefore, this study aimed to examine the effects of liver fibrosis on hydrodynamic gene delivery efficiency using a rat liver fibrosis model. We demonstrated for the first time, using pCMV-Luc plasmid, that this procedure is safe and that the amount of fibrotic tissue in the liver decreases gene delivery efficiency, resulting in decrease in luciferase activity depending on the volume of fibrotic tissue in the liver and the number of hepatocytes that are immunohistochemically stained positive for transgene product. We further demonstrate that antifibrotic gene therapy with matrix metalloproteinase-13 gene reduces liver fibrosis and improves efficiency of hydrodynamic gene delivery. These results demonstrate the negative effects of fibrotic tissue on hydrodynamic gene delivery and its recovery by appropriate antifibrotic therapy. äEffects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Deliver(epmc).pdf DeepDyve Pubget Overpricing