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10.1038/srep33235 http://scihub22266oqcxt.onion/10.1038/srep33235 C5021930!5021930!27624517     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=27624517|t=2016. The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7 |pdf=|usr=}}{{27624517}} gab.com Text The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7 JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27624517 #FOAvip Rapid detection of the foodborne pathogen Escherichia coli O157:H7 is of vital importance for public health worldwide. Among detection methods, reporter phages represent unique and sensitive tools for the detection of E. coli O157:H7 from food as they are host-specific and able to differentiate live cells from dead ones. Upon infection, target bacteria become identifiable since reporter genes are expressed from the engineered phage genome. The E. coli O157:H7 bacteriophage ?V10 was modified to express NanoLuc luciferase (Nluc) derived from the deep-sea shrimp Oplophorus gracilirostris. Once infected by the ?V10 reporter phage, E. coli O157:H7 produces a strong bioluminescent signal upon addition of commercial luciferin (Nano-Glo®). Enrichment assays using E. coli O157:H7 grown in LB broth with a reporter phage concentration of 1.76?×?102?pfu ml?1 are capable of detecting approximately 5 CFU in 7?hours. Comparable detection was achieved within 9?hours using 9.23?×?103?pfu ml?1 of phage in selective culture enrichments of ground beef as a representative food matrix. Therefore we conclude that this NanoLuc reporter phage assay shows promise for detection of E. coli O157:H7 from food in a simple, fast and sensitive manner. Twit Text FOAVip The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7 ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27624517 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27624517 #FOAvip English Wikipedia <ref>{{Cite pmid|27624517}}</ref> The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7 #MMPMID27624517 Zhang D; Coronel-Aguilera CP; Romero PL; Perry L; Minocha U; Rosenfield C; Gehring AG; Paoli GC; Bhunia AK; Applegate B Sci Rep 2016[]; 6 (ä): ä PMID27624517show ga Rapid detection of the foodborne pathogen Escherichia coli O157:H7 is of vital importance for public health worldwide. Among detection methods, reporter phages represent unique and sensitive tools for the detection of E. coli O157:H7 from food as they are host-specific and able to differentiate live cells from dead ones. Upon infection, target bacteria become identifiable since reporter genes are expressed from the engineered phage genome. The E. coli O157:H7 bacteriophage ?V10 was modified to express NanoLuc luciferase (Nluc) derived from the deep-sea shrimp Oplophorus gracilirostris. Once infected by the ?V10 reporter phage, E. coli O157:H7 produces a strong bioluminescent signal upon addition of commercial luciferin (Nano-Glo®). Enrichment assays using E. coli O157:H7 grown in LB broth with a reporter phage concentration of 1.76?×?102?pfu ml?1 are capable of detecting approximately 5 CFU in 7?hours. Comparable detection was achieved within 9?hours using 9.23?×?103?pfu ml?1 of phage in selective culture enrichments of ground beef as a representative food matrix. Therefore we conclude that this NanoLuc reporter phage assay shows promise for detection of E. coli O157:H7 from food in a simple, fast and sensitive manner. äThe Use of a Novel NanoLuc -Based Reporter Phage for the Detection of (epmc).pdf DeepDyve Pubget Overpricing