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10.3389/fmicb.2016.01451 http://scihub22266oqcxt.onion/10.3389/fmicb.2016.01451 C5020066/?report=reader!5020066!27679622     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Microbiol 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=27679622|t=2016. Development of a Lateral Flow Immunoassay for the Rapid Diagnosis of Invasive Candidiasis |pdf=|usr=}}{{27679622}} gab.com Text Development of a Lateral Flow Immunoassay for the Rapid Diagnosis of Invasive Candidiasis JO: Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27679622 #FOAvip Early and accurate diagnosis of invasive candidiasis (IC) is very important. In this study, a lateral flow immunoassay (LFIA) was developed to detect antibody against Candida albicans enolase (Eno). Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L) was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L) and goat anti IgG (1.0 mg/L) were immobilized in test and control lines, respectively, of a nitrocellulose membrane, acting as the capture reagents. The LFIA was used to detect anti Eno in 38 sera from clinically proven IC patients, as well as in 50 healthy control subjects. Compared with an indirect ELISA designed as a reference test, the specificity and sensitivity of the LFIA were 98.2 and 84.8%, respectively. Excellent agreement between the results obtained by ELISA and the LFIA (? = 0.851) was observed in this study. In addition, the agreement between the blood culture results and LFIA test is strong (? = 0.658). The data presented in the study indicate that the LFIA test is a suitable tool for the serological surveillance of IC in the field or in poorly equipped laboratories. Twit Text FOAVip Development of a Lateral Flow Immunoassay for the Rapid Diagnosis of Invasive Candidiasis ????Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27679622 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27679622 #FOAvip English Wikipedia <ref>{{Cite pmid|27679622}}</ref> Development of a Lateral Flow Immunoassay for the Rapid Diagnosis of Invasive Candidiasis #MMPMID27679622 He ZX; Shi LC; Ran XY; Li W; Wang XL; Wang FK Front Microbiol 2016[]; 7 (ä): ä PMID27679622show ga Early and accurate diagnosis of invasive candidiasis (IC) is very important. In this study, a lateral flow immunoassay (LFIA) was developed to detect antibody against Candida albicans enolase (Eno). Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L) was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L) and goat anti IgG (1.0 mg/L) were immobilized in test and control lines, respectively, of a nitrocellulose membrane, acting as the capture reagents. The LFIA was used to detect anti Eno in 38 sera from clinically proven IC patients, as well as in 50 healthy control subjects. Compared with an indirect ELISA designed as a reference test, the specificity and sensitivity of the LFIA were 98.2 and 84.8%, respectively. Excellent agreement between the results obtained by ELISA and the LFIA (? = 0.851) was observed in this study. In addition, the agreement between the blood culture results and LFIA test is strong (? = 0.658). The data presented in the study indicate that the LFIA test is a suitable tool for the serological surveillance of IC in the field or in poorly equipped laboratories. äDevelopment of a Lateral Flow Immunoassay for the Rapid Diagnosis of I(epmc).pdf DeepDyve Pubget Overpricing