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10.1039/C6RA01546H http://scihub22266oqcxt.onion/10.1039/C6RA01546H C5019568!5019568!27642512     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RSC+Adv 2016 ; 48 (6): 41665-74 Nephropedia Template TP {{tp|p=27642512|t=2016. Enhancing Intracranial Delivery of Clinically Relevant Non-viral Gene Vectors |pdf=|usr=}}{{27642512}} gab.com Text Enhancing Intracranial Delivery of Clinically Relevant Non-viral Gene Vectors JO: RSC Adv http://www.kidney.de/pget.php?&tw=1&pmid=27642512 #FOAvip Gene therapy is a promising strategy for the management of various neurological disorders that do not respond adequately to conventional therapeutics. The development of gene vectors with favorable safety profiles that can achieve uniform distribution and high-level transgene expression in the brain remains challenging. The rod-shaped, non-viral gene delivery platform based on poly-L-lysine (PLL) conjugated to a single segment of polyethylene glycol (PEG) has shown safe transfection in human nares and mouse brains in vivo. However, we have previously demonstrated that a denser PEG coating is required for rapid diffusion of nanoparticles in the brain extracellular space. Here, we engineered a densely PEGylated version of this platform based on PLL polymers conjugated to branched PEG via alkyne-azide cycloaddition. We found that the newly developed gene vectors rapidly diffused in the brain parenchyma, providing significantly improved vector distribution and overall transgene expression in vivo compared to the previously developed platform. These brain-penetrating DNA nanoparticles exhibited enhanced cellular uptake presumably due to their ellipsoidal morphology. By simultaneously improving delivery to target cells and subsequent transfection, our densely PEGylated PLL DNA nanoparticles can provide widespread, high levels of transgene expression, essential for effective targeting of highly disseminated brain diseases. Twit Text FOAVip Enhancing Intracranial Delivery of Clinically Relevant Non-viral Gene Vectors ????RSC Adv http://www.kidney.de/pget.php?&tw=1&pmid=27642512 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27642512 #FOAvip English Wikipedia <ref>{{Cite pmid|27642512}}</ref> Enhancing Intracranial Delivery of Clinically Relevant Non-viral Gene Vectors #MMPMID27642512 Berry S; Mastorakos P; Zhang C; Song E; Patel H; Suk JS; Hanes J RSC Adv 2016[]; 48 (6): 41665-74 PMID27642512show ga Gene therapy is a promising strategy for the management of various neurological disorders that do not respond adequately to conventional therapeutics. The development of gene vectors with favorable safety profiles that can achieve uniform distribution and high-level transgene expression in the brain remains challenging. The rod-shaped, non-viral gene delivery platform based on poly-L-lysine (PLL) conjugated to a single segment of polyethylene glycol (PEG) has shown safe transfection in human nares and mouse brains in vivo. However, we have previously demonstrated that a denser PEG coating is required for rapid diffusion of nanoparticles in the brain extracellular space. Here, we engineered a densely PEGylated version of this platform based on PLL polymers conjugated to branched PEG via alkyne-azide cycloaddition. We found that the newly developed gene vectors rapidly diffused in the brain parenchyma, providing significantly improved vector distribution and overall transgene expression in vivo compared to the previously developed platform. These brain-penetrating DNA nanoparticles exhibited enhanced cellular uptake presumably due to their ellipsoidal morphology. By simultaneously improving delivery to target cells and subsequent transfection, our densely PEGylated PLL DNA nanoparticles can provide widespread, high levels of transgene expression, essential for effective targeting of highly disseminated brain diseases. äEnhancing Intracranial Delivery of Clinically Relevant Non-viral Gene (epmc).pdf DeepDyve Pubget Overpricing