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2016 ; 18
(8
): 459-67
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High-Resolution Multispectral Optoacoustic Tomography of the Vascularization and
Constitutive Hypoxemia of Cancerous Tumors
#MMPMID27566102
Chekkoury A
; Nunes A
; Gateau J
; Symvoulidis P
; Feuchtinger A
; Beziere N
; Ovsepian SV
; Walch A
; Ntziachristos V
Neoplasia
2016[Aug]; 18
(8
): 459-67
PMID27566102
show ga
Diversity of the design and alignment of illumination and ultrasonic transducers
empower the fine scalability and versatility of optoacoustic imaging. In this
study, we implement an innovative high-resolution optoacoustic mesoscopy for
imaging the vasculature and tissue oxygenation within subcutaneous and orthotopic
cancerous implants of mice in vivo through acquisition of tomographic projections
over 180° at a central frequency of 24 MHz. High-resolution volumetric imaging
was combined with multispectral functional measurements to resolve the exquisite
inner structure and vascularization of the entire tumor mass using endogenous and
exogenous optoacoustic contrast. Evidence is presented for constitutive hypoxemia
within the carcinogenic tissue through analysis of the hemoglobin absorption
spectra and distribution. Morphometric readouts obtained with optoacoustic
mesoscopy have been verified with high-resolution ultramicroscopic studies. The
findings described herein greatly extend the applications of optoacoustic
mesoscopy toward structural and multispectral functional measurements of the
vascularization and hemodynamics within solid tumors in vivo and are of major
relevance to basic and preclinical oncological studies in small animal models.