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10.1016/j.cell.2016.05.032 http://scihub22266oqcxt.onion/10.1016/j.cell.2016.05.032 C5017859!5017859!27293192     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2016 ; 166 (1): 102-14 Nephropedia Template TP {{tp|p=27293192|t=2016. Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination |pdf=|usr=}}{{27293192}} gab.com Text Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=27293192 #FOAvip Co-option of RAG1 and RAG2 for antigen receptor gene assembly by V(D)J recombination was a crucial event in the evolution of jawed vertebrate adaptive immunity. RAG1/2 are proposed to have arisen from a transposable element, but definitive evidence for this is lacking. Here we report the discovery of ProtoRAG, a DNA transposon family from lancelets, the most basal extant chordates. A typical ProtoRAG is flanked by 5 bp target site duplications and a pair of terminal inverted repeats (TIRs) resembling V(D)J recombination signal sequences. Between the TIRs reside tail-to-tail oriented, intron-containing RAG1-like and RAG2-like genes. We demonstrate that ProtoRAG was recently active in the lancelet germline and that the lancelet RAG1/2-like proteins can mediate TIR-dependent transposon excision, host DNA recombination, transposition, and low efficiency TIR rejoining using reaction mechanisms similar to those used by vertebrate RAGs. We propose that ProtoRAG represents a molecular ?living fossil? of the long-sought RAG transposon. Twit Text FOAVip Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=27293192 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27293192 #FOAvip English Wikipedia <ref>{{Cite pmid|27293192}}</ref> Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination #MMPMID27293192 Huang S; Tao X; Yuan S; Zhang Y; Li P; Beilinson HA; Zhang Y; Yu W; Pontarotti P; Escriva H; Le Petillon Y; Liu X; Chen S; Schatz DG; Xu A Cell 2016[Jun]; 166 (1): 102-14 PMID27293192show ga Co-option of RAG1 and RAG2 for antigen receptor gene assembly by V(D)J recombination was a crucial event in the evolution of jawed vertebrate adaptive immunity. RAG1/2 are proposed to have arisen from a transposable element, but definitive evidence for this is lacking. Here we report the discovery of ProtoRAG, a DNA transposon family from lancelets, the most basal extant chordates. A typical ProtoRAG is flanked by 5 bp target site duplications and a pair of terminal inverted repeats (TIRs) resembling V(D)J recombination signal sequences. Between the TIRs reside tail-to-tail oriented, intron-containing RAG1-like and RAG2-like genes. We demonstrate that ProtoRAG was recently active in the lancelet germline and that the lancelet RAG1/2-like proteins can mediate TIR-dependent transposon excision, host DNA recombination, transposition, and low efficiency TIR rejoining using reaction mechanisms similar to those used by vertebrate RAGs. We propose that ProtoRAG represents a molecular ?living fossil? of the long-sought RAG transposon. äDiscovery of an Active RAG Transposon Illuminates the Origins of V(D)J(epmc).pdf DeepDyve Pubget Overpricing