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2016 ; 2016
(ä): 4896319
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The Clinical and Medicolegal Analysis of Electrical Shocked Rats: Based on the
Serological and Histological Methods
#MMPMID27648446
Liu H
; Wang Q
; Zhao Z
; Xie Y
; Ding S
; Wang Z
Biomed Res Int
2016[]; 2016
(ä): 4896319
PMID27648446
show ga
This research was aimed at discovering the serological and histological changes
in cardiac and hepatic tissue after electric shock. The CK-MB, ALT, and AMS
indexes were tested with serological methods. Moreover, the Bcl-2, Bax, and
Hsp-60 expression levels were carefully measured. An electrical injury model was
established by giving rats electric shocks at 110?V with alternating electric
current. Blood samples from the rats were analyzed for the biochemical indexes.
The degrees of pathological changes in the heart and liver were evaluated using
IHC staining for Bcl-2, Bax, and Hsp-60. The levels of CK-MB in the electrical
injury group rapidly peaked at 0.5 hours after the electric shock. Additionally,
the levels of Bcl-2, Bax, and Hsp-60 in the cardiac and hepatic tissues changed
regularly after the electrical injury and exhibited apparent differences from the
levels in the control group. CK-MB, ALT, and AMS were altered regularly after
electric shock, and these results provide significant information for clinical
and medicolegal practice. This research has shed light on the assessment of
electrical injury without obvious electrical burns. Furthermore, the findings
obtained for Bcl-2/Bax and Hsp-60 can also facilitate pathological diagnosis and
the identification of antemortem and postmortem electrical injury.