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10.1111/jeu.12338 http://scihub22266oqcxt.onion/10.1111/jeu.12338 C5012946!5012946!27315329     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Eukaryot+Microbiol 2016 ; 63 (5): 679-90 Nephropedia Template TP {{tp|p=27315329|t=2016. Genome Editing by CRISPR/Cas9: a Game Change in the Genetic Manipulation of Protists |pdf=|usr=}}{{27315329}} gab.com Text Genome Editing by CRISPR/Cas9: a Game Change in the Genetic Manipulation of Protists JO: J Eukaryot Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27315329 #FOAvip Genome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system has been transformative in biology. Originally discovered as an adaptive prokaryotic immune system, CRISPR/Cas9 has been repurposed for genome editing in a broad range of model organisms, from yeast to mammalian cells. Protist parasites are unicellular organisms producing important human diseases that affect millions of people around the world. For many of these diseases, such as malaria, Chagas disease, leishmaniasis and cryptosporidiosis, there are no effective treatments or vaccines available. The recent adaptation of the CRISPR/Cas9 technology to several protist models will be playing a key role in the functional study of their proteins, in the characterization of their metabolic pathways, and in the understanding of their biology, and will facilitate the search for new chemotherapeutic targets. In this work we review recent studies where the CRISPR/Cas9 system was adapted to protist parasites, particularly to Apicomplexans and trypanosomatids, emphasizing the different molecular strategies used for genome editing of each organism, as well as their advantages. We also discuss the potential usefulness of this technology in the green alga Chlamydomonas reinhardtii. Twit Text FOAVip Genome Editing by CRISPR/Cas9: a Game Change in the Genetic Manipulation of Protists ????J Eukaryot Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27315329 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27315329 #FOAvip English Wikipedia <ref>{{Cite pmid|27315329}}</ref> Genome Editing by CRISPR/Cas9: a Game Change in the Genetic Manipulation of Protists #MMPMID27315329 Lander N; Chiurillo MA; Docampo R J Eukaryot Microbiol 2016[Sep]; 63 (5): 679-90 PMID27315329show ga Genome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system has been transformative in biology. Originally discovered as an adaptive prokaryotic immune system, CRISPR/Cas9 has been repurposed for genome editing in a broad range of model organisms, from yeast to mammalian cells. Protist parasites are unicellular organisms producing important human diseases that affect millions of people around the world. For many of these diseases, such as malaria, Chagas disease, leishmaniasis and cryptosporidiosis, there are no effective treatments or vaccines available. The recent adaptation of the CRISPR/Cas9 technology to several protist models will be playing a key role in the functional study of their proteins, in the characterization of their metabolic pathways, and in the understanding of their biology, and will facilitate the search for new chemotherapeutic targets. In this work we review recent studies where the CRISPR/Cas9 system was adapted to protist parasites, particularly to Apicomplexans and trypanosomatids, emphasizing the different molecular strategies used for genome editing of each organism, as well as their advantages. We also discuss the potential usefulness of this technology in the green alga Chlamydomonas reinhardtii. äGenome Editing by CRISPR/Cas9: a Game Change in the Genetic Manipulati(epmc).pdf DeepDyve Pubget Overpricing