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10.1186/s13578-016-0118-2 http://scihub22266oqcxt.onion/10.1186/s13578-016-0118-2 C5012070!5012070!27602203     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Biosci 2016 ; 6 (1): ä Nephropedia Template TP {{tp|p=27602203|t=2016. A new paradigm of DNA synthesis: three-metal-ion catalysis |pdf=|usr=}}{{27602203}} gab.com Text A new paradigm of DNA synthesis: three-metal-ion catalysis JO: Cell Biosci http://www.kidney.de/pget.php?&tw=1&pmid=27602203 #FOAvip Enzyme catalysis has been studied for over a century. How it actually occurs has not been visualized until recently. By combining in crystallo reaction and X-ray diffraction analysis of reaction intermediates, we have obtained unprecedented atomic details of the DNA synthesis process. Contrary to the established theory that enzyme-substrate complexes and transition states have identical atomic composition and catalysis occurs by the two-metal-ion mechanism, we have discovered that an additional divalent cation has to be captured en route to product formation. Unlike the canonical two metal ions, which are coordinated by DNA polymerases, this third metal ion is free of enzyme coordination. Its location between the ?- and ?-phosphates of dNTP suggests that the third metal ion may drive the phosphoryltransfer from the leaving group opposite to the 3?-OH nucleophile. Experimental data indicate that binding of the third metal ion may be the rate-limiting step in DNA synthesis and the free energy associated with the metal-ion binding can overcome the activation barrier to the DNA synthesis reaction.Electronic supplementary material: The online version of this article (doi:10.1186/s13578-016-0118-2) contains supplementary material, which is available to authorized users. Twit Text FOAVip A new paradigm of DNA synthesis: three-metal-ion catalysis ????Cell Biosci http://www.kidney.de/pget.php?&tw=1&pmid=27602203 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27602203 #FOAvip English Wikipedia <ref>{{Cite pmid|27602203}}</ref> A new paradigm of DNA synthesis: three-metal-ion catalysis #MMPMID27602203 Yang W; Weng PJ; Gao Y Cell Biosci 2016[]; 6 (1): ä PMID27602203show ga Enzyme catalysis has been studied for over a century. How it actually occurs has not been visualized until recently. By combining in crystallo reaction and X-ray diffraction analysis of reaction intermediates, we have obtained unprecedented atomic details of the DNA synthesis process. Contrary to the established theory that enzyme-substrate complexes and transition states have identical atomic composition and catalysis occurs by the two-metal-ion mechanism, we have discovered that an additional divalent cation has to be captured en route to product formation. Unlike the canonical two metal ions, which are coordinated by DNA polymerases, this third metal ion is free of enzyme coordination. Its location between the ?- and ?-phosphates of dNTP suggests that the third metal ion may drive the phosphoryltransfer from the leaving group opposite to the 3?-OH nucleophile. Experimental data indicate that binding of the third metal ion may be the rate-limiting step in DNA synthesis and the free energy associated with the metal-ion binding can overcome the activation barrier to the DNA synthesis reaction.Electronic supplementary material: The online version of this article (doi:10.1186/s13578-016-0118-2) contains supplementary material, which is available to authorized users. äA new paradigm of DNA synthesis: three-metal-ion catalysis (epmc).pdf DeepDyve Pubget Overpricing