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10.4049/jimmunol.1600180 http://scihub22266oqcxt.onion/10.4049/jimmunol.1600180 C5010990!5010990!27534551     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol 2016 ; 197 (6): 2090-101 Nephropedia Template TP {{tp|p=27534551|t=2016. Dynein separately partners with NDE1 and dynactin to orchestrate T cell focused secretion |pdf=|usr=}}{{27534551}} gab.com Text Dynein separately partners with NDE1 and dynactin to orchestrate T cell focused secretion JO: J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27534551 #FOAvip Helper and cytotoxic T cells accomplish focused secretion through the clustering of vesicles around the MTOC and translocation of the MTOC to the target contact site. Here, using Jurkat cells and OT-I T cell receptor (TcR) transgenic primary murine CTLs, we show that the dynein-binding proteins NDE1 and dynactin (as represented by p150Glued) form mutually exclusive complexes with dynein, exhibit non-overlapping distributions in target-stimulated cells, and mediate different transport events. When Jurkat cells expressing a dominant negative form of NDE1 (NDE1-EGFP fusion) were activated by SEE-coated Raji cells, NDE1 and dynein failed to accumulate at the immunological synapse (IS) and MTOC translocation was inhibited. Knockdown of NDE1 in Jurkat cells or primary mouse CTLs also inhibited MTOC translocation and CTL-mediated killing. In contrast to NDE1, knockdown of p150Glued, which depleted the alternative dynein-dynactin complex, resulted in impaired accumulation of CTLA-4 and granzyme-B containing intracellular vesicles at the IS, while MTOC translocation was not affected. Depletion of p150Glued in CTLs also inhibited CTL-mediated lysis. We conclude that the NDE1/Lis1 and dynactin complexes separately mediate two key components of T cell focused secretion, namely translocation of the MTOC and lytic granules to the IS, respectively. Twit Text FOAVip Dynein separately partners with NDE1 and dynactin to orchestrate T cell focused secretion ????J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27534551 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27534551 #FOAvip English Wikipedia <ref>{{Cite pmid|27534551}}</ref> Dynein separately partners with NDE1 and dynactin to orchestrate T cell focused secretion #MMPMID27534551 Nath S; Christian L; Tan SY; Ki S; Ehrlich LIR; Poenie M J Immunol 2016[Sep]; 197 (6): 2090-101 PMID27534551show ga Helper and cytotoxic T cells accomplish focused secretion through the clustering of vesicles around the MTOC and translocation of the MTOC to the target contact site. Here, using Jurkat cells and OT-I T cell receptor (TcR) transgenic primary murine CTLs, we show that the dynein-binding proteins NDE1 and dynactin (as represented by p150Glued) form mutually exclusive complexes with dynein, exhibit non-overlapping distributions in target-stimulated cells, and mediate different transport events. When Jurkat cells expressing a dominant negative form of NDE1 (NDE1-EGFP fusion) were activated by SEE-coated Raji cells, NDE1 and dynein failed to accumulate at the immunological synapse (IS) and MTOC translocation was inhibited. Knockdown of NDE1 in Jurkat cells or primary mouse CTLs also inhibited MTOC translocation and CTL-mediated killing. In contrast to NDE1, knockdown of p150Glued, which depleted the alternative dynein-dynactin complex, resulted in impaired accumulation of CTLA-4 and granzyme-B containing intracellular vesicles at the IS, while MTOC translocation was not affected. Depletion of p150Glued in CTLs also inhibited CTL-mediated lysis. We conclude that the NDE1/Lis1 and dynactin complexes separately mediate two key components of T cell focused secretion, namely translocation of the MTOC and lytic granules to the IS, respectively. äDynein separately partners with NDE1 and dynactin to orchestrate T cel(epmc).pdf DeepDyve Pubget Overpricing