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10.1093/gbe/evw159

http://scihub22266oqcxt.onion/10.1093/gbe/evw159
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suck abstract from ncbi


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pmid27389686
      Genome+Biol+Evol 2016 ; 8 (8 ): 2406-12
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  • When Genomics Is Not Enough: Experimental Evidence for a Decrease in LINE-1 Activity During the Evolution of Australian Marsupials #MMPMID27389686
  • Gallus S ; Lammers F ; Nilsson MA
  • Genome Biol Evol 2016[Aug]; 8 (8 ): 2406-12 PMID27389686 show ga
  • The autonomous transposable element LINE-1 is a highly abundant element that makes up between 15% and 20% of therian mammal genomes. Since their origin before the divergence of marsupials and placental mammals, LINE-1 elements have contributed actively to the genome landscape. A previous in silico screen of the Tasmanian devil genome revealed a lack of functional coding LINE-1 sequences. In this study we present the results of an in vitro analysis from a partial LINE-1 reverse transcriptase coding sequence in five marsupial species. Our experimental screen supports the in silico findings of the genome-wide degradation of LINE-1 sequences in the Tasmanian devil, and identifies a high frequency of degraded LINE-1 sequences in other Australian marsupials. The comparison between the experimentally obtained LINE-1 sequences and reference genome assemblies suggests that conclusions from in silico analyses of retrotransposition activity can be influenced by incomplete genome assemblies from short reads.
  • |*Evolution, Molecular [MESH]
  • |*Phylogeny [MESH]
  • |Animals [MESH]
  • |Australia [MESH]
  • |Computer Simulation [MESH]
  • |Genomics [MESH]
  • |Long Interspersed Nucleotide Elements/*genetics [MESH]
  • |Mammals/genetics [MESH]


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