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10.1038/ncomms12524

http://scihub22266oqcxt.onion/10.1038/ncomms12524
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C5007439!5007439!27561669
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suck abstract from ncbi


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pmid27561669      Nat+Commun 2016 ; 7 (ä): ä
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  • Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ #MMPMID27561669
  • Koning RI; Gomez-Blanco J; Akopjana I; Vargas J; Kazaks A; Tars K; Carazo JM; Koster AJ
  • Nat Commun 2016[]; 7 (ä): ä PMID27561669show ga
  • In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome. This reveals that in situ, the viral RNA genome can adopt a defined conformation. The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly.
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