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10.1369/0022155416659249 http://scihub22266oqcxt.onion/10.1369/0022155416659249 C5006137!5006137 !27449741     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27449741 &cmd=llinks): Failed to open stream: HTTP request failed! 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Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples |pdf=|usr=}}{{27449741 }} gab.com Text Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples JO: J Histochem Cytochem http://www.kidney.de/pget.php?&tw=1&pmid=27449741 #FOAvip Stromal collagen alignment has been shown to have clinical significance in a variety of cancers and in other diseases accompanied by fibrosis. While much of the biological and clinical importance of collagen changes has been demonstrated using second harmonic generation (SHG) imaging in experimental settings, implementation into routine clinical pathology practice is currently prohibitive. To translate the assessment of collagen organization into routine pathology workflow, a surrogate visualization method needs to be examined. The objective of the present study was to quantitatively compare collagen metrics generated from SHG microscopy and commonly available picrosirius red stain with standard polarization microscopy (PSR-POL). Each technique was quantitatively compared with established image segmentation and fiber tracking algorithms using human pancreatic cancer as a model, which is characterized by a pronounced stroma with reorganized collagen fibers. Importantly, PSR-POL produced similar quantitative trends for most collagen metrics in benign and cancerous tissues as measured by SHG. We found it notable that PSR-POL detects higher fiber counts, alignment, length, straightness, and width compared with SHG imaging but still correlates well with SHG results. PSR-POL may provide sufficient and additional information in a conventional clinical pathology laboratory for certain types of collagen quantification. Twit Text FOAVip Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples ????J Histochem Cytochem http://www.kidney.de/pget.php?&tw=1&pmid=27449741 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27449741 #FOAvip English Wikipedia <ref>{{Cite pmid|27449741 }}</ref> Comparison of Picrosirius Red Staining With Second Harmonic Generation Imaging for the Quantification of Clinically Relevant Collagen Fiber Features in Histopathology Samples #MMPMID27449741 Drifka CR ; Loeffler AG ; Mathewson K ; Mehta G ; Keikhosravi A ; Liu Y ; Lemancik S ; Ricke WA ; Weber SM ; Kao WJ ; Eliceiri KW J Histochem Cytochem 2016[Sep]; 64 (9 ): 519-29 PMID27449741 show ga Stromal collagen alignment has been shown to have clinical significance in a variety of cancers and in other diseases accompanied by fibrosis. While much of the biological and clinical importance of collagen changes has been demonstrated using second harmonic generation (SHG) imaging in experimental settings, implementation into routine clinical pathology practice is currently prohibitive. To translate the assessment of collagen organization into routine pathology workflow, a surrogate visualization method needs to be examined. The objective of the present study was to quantitatively compare collagen metrics generated from SHG microscopy and commonly available picrosirius red stain with standard polarization microscopy (PSR-POL). Each technique was quantitatively compared with established image segmentation and fiber tracking algorithms using human pancreatic cancer as a model, which is characterized by a pronounced stroma with reorganized collagen fibers. Importantly, PSR-POL produced similar quantitative trends for most collagen metrics in benign and cancerous tissues as measured by SHG. We found it notable that PSR-POL detects higher fiber counts, alignment, length, straightness, and width compared with SHG imaging but still correlates well with SHG results. PSR-POL may provide sufficient and additional information in a conventional clinical pathology laboratory for certain types of collagen quantification. |Azo Compounds/*chemistry [MESH] |Coloring Agents/*chemistry [MESH] |Fibrillar Collagens/*analysis [MESH] |Humans [MESH] |Microscopy/methods [MESH] |Pancreatic Neoplasms/*chemistry [MESH]Comparison of Picrosirius Red Staining With Second Harmonic Generation(epmc).pdf DeepDyve Pubget Overpricing