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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Clin+Microbiol
2016 ; 54
(9
): 2373-9
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Moraxella catarrhalis Might Be More Common than Expected in Acute Otitis Media in
Young Finnish Children
#MMPMID27413187
Sillanpää S
; Oikarinen S
; Sipilä M
; Kramna L
; Rautiainen M
; Huhtala H
; Aittoniemi J
; Laranne J
; Hyöty H
; Cinek O
J Clin Microbiol
2016[Sep]; 54
(9
): 2373-9
PMID27413187
show ga
According to studies based on bacterial cultures of middle ear fluids,
Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis have
been the most common pathogens in acute otitis media. However, bacterial culture
can be affected by reduced viability or suboptimal growth of bacteria. PCR
detects bacterial DNA from samples with greater sensitivity than culture. In the
present study, we analyzed the middle ear pathogens with both conventional
culture and semiquantitative real-time PCR in 90 middle ear fluid samples
obtained from children aged 5 to 42 months during acute otitis media episodes.
Samples were tested for the presence of S. pneumoniae, H. influenzae, M.
catarrhalis, Alloiococcus otitidis, Staphylococcus aureus, and Pseudomonas
aeruginosa One or more bacterial pathogens were detected in 42 (47%) samples with
culture and in 69 (77%) samples with PCR. According to PCR analysis, M.
catarrhalis results were positive in 42 (47%) samples, H. influenzae in 30 (33%),
S. pneumoniae in 27 (30%), A. otitidis in 6 (6.7%), S. aureus in 5 (5.6%), and P.
aeruginosa in 1 (1.1%). Multibacterial etiology was seen in 34 (38%) samples, and
M. catarrhalis was detected in most (85%) of those cases. Fifteen signals for M.
catarrhalis were strong, suggesting a highly probable etiological role of the
pathogen. In conclusion, even though M. catarrhalis is often a part of mixed
flora in acute otitis media, a considerable proportion of cases may be primarily
attributable to this pathogen.