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10.1016/j.cell.2016.06.016

http://scihub22266oqcxt.onion/10.1016/j.cell.2016.06.016
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C5005454!5005454!27397506
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suck abstract from ncbi

pmid27397506      Cell 2016 ; 166 (4): 1028-40
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  • Ultra-High Resolution 3D Imaging of Whole Cells #MMPMID27397506
  • Huang F; Sirinakis G; Allgeyer E; Schroeder L; Duim W; Kromann E; Phan T; Rivera-Molina F; Myers J; Irnov I; Lessard M; Zhang Y; Handel M; Jacobs-Wagner C; Lusk C; Rothman J; Toomre D; Booth M; Bewersdorf J
  • Cell 2016[Aug]; 166 (4): 1028-40 PMID27397506show ga
  • Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50?80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.
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