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10.3389/fimmu.2016.00326 http://scihub22266oqcxt.onion/10.3389/fimmu.2016.00326 C5003846!5003846 !27625650     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27625650 &cmd=llinks): Failed to open stream: HTTP request failed! 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A Well-Controlled Experimental System to Study Interactions of Cytotoxic T Lymphocytes with Tumor Cells |pdf=|usr=}}{{27625650 }} gab.com Text A Well-Controlled Experimental System to Study Interactions of Cytotoxic T Lymphocytes with Tumor Cells JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27625650 #FOAvip While T cell-based immunotherapies are steadily improving, there are still many patients who progress, despite T cell-infiltrated tumors. Emerging evidence suggests that T cells themselves may provoke immune escape of cancer cells. Here, we describe a well-controlled co-culture system for studying the dynamic T cell - cancer cell interplay, using human melanoma as a model. We explain starting material, controls, and culture parameters to establish reproducible and comparable cultures with highly heterogeneous tumor cells. Low passage melanoma cell lines and melanoma-specific CD8+ T cell clones generated from patient blood were cultured together for up to 3?days. Living melanoma cells were isolated from the co-culture system by fluorescence-activated cell sorting. We demonstrate that the characterization of isolated melanoma cells is feasible using flow cytometry for protein expression analysis as well as an Agilent whole human genome microarray and the NanoString technology for differential gene expression analysis. In addition, we identify five genes (ALG12, GUSB, RPLP0, KRBA2, and ADAT2) that are stably expressed in melanoma cells independent of the presence of T cells or the T cell-derived cytokines IFN? and TNF?. These genes are essential for correct normalization of gene expression data by NanoString. Further to the characterization of melanoma cells after exposure to CTLs, this experimental system might be suitable to answer a series of questions, including how the affinity of CTLs for their target antigen influences the melanoma cell response and whether CTL-induced gene expression changes in melanoma cells are reversible. Taken together, our human T cell - melanoma cell culture system is well suited to characterize immune-related mechanisms in cancer cells. Twit Text FOAVip A Well-Controlled Experimental System to Study Interactions of Cytotoxic T Lymphocytes with Tumor Cells ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27625650 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27625650 #FOAvip English Wikipedia <ref>{{Cite pmid|27625650 }}</ref> A Well-Controlled Experimental System to Study Interactions of Cytotoxic T Lymphocytes with Tumor Cells #MMPMID27625650 Neubert NJ ; Soneson C ; Barras D ; Baumgaertner P ; Rimoldi D ; Delorenzi M ; Fuertes Marraco SA ; Speiser DE Front Immunol 2016[]; 7 (ä): 326 PMID27625650 show ga While T cell-based immunotherapies are steadily improving, there are still many patients who progress, despite T cell-infiltrated tumors. Emerging evidence suggests that T cells themselves may provoke immune escape of cancer cells. Here, we describe a well-controlled co-culture system for studying the dynamic T cell - cancer cell interplay, using human melanoma as a model. We explain starting material, controls, and culture parameters to establish reproducible and comparable cultures with highly heterogeneous tumor cells. Low passage melanoma cell lines and melanoma-specific CD8+ T cell clones generated from patient blood were cultured together for up to 3?days. Living melanoma cells were isolated from the co-culture system by fluorescence-activated cell sorting. We demonstrate that the characterization of isolated melanoma cells is feasible using flow cytometry for protein expression analysis as well as an Agilent whole human genome microarray and the NanoString technology for differential gene expression analysis. In addition, we identify five genes (ALG12, GUSB, RPLP0, KRBA2, and ADAT2) that are stably expressed in melanoma cells independent of the presence of T cells or the T cell-derived cytokines IFN? and TNF?. These genes are essential for correct normalization of gene expression data by NanoString. Further to the characterization of melanoma cells after exposure to CTLs, this experimental system might be suitable to answer a series of questions, including how the affinity of CTLs for their target antigen influences the melanoma cell response and whether CTL-induced gene expression changes in melanoma cells are reversible. Taken together, our human T cell - melanoma cell culture system is well suited to characterize immune-related mechanisms in cancer cells. äA Well-Controlled Experimental System to Study Interactions of Cytotox(epmc).pdf DeepDyve Pubget Overpricing