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10.1016/j.jneumeth.2016.07.003 http://scihub22266oqcxt.onion/10.1016/j.jneumeth.2016.07.003 C5003758!5003758!27393311     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Neurosci+Methods 2016 ; 271 (ä): 55-64 Nephropedia Template TP {{tp|p=27393311|t=2016. Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets |pdf=|usr=}}{{27393311}} gab.com Text Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets JO: J Neurosci Methods http://www.kidney.de/pget.php?&tw=1&pmid=27393311 #FOAvip Background: Marmosets are a powerful, emerging model for human behavior and neurological disorders. However, longitudinal imaging modalities that visualize both cellular structure and function within the cortex are not available in this animal model. Hence, we implemented an approach to quantify vascular topology, hemodynamics, and neural activity in awake marmosets using two-photon microscopy (2PM). New method: Marmosets were acclimated to a custom stereotaxic system. AAV1-GCaMP5G was injected into somatosensory cortex to optically indicate neural activity, and a cranial chamber was implanted. Results: Longitudinal 2PM revealed vasculature and neurons 500 µm below the cortical surface. Vascular response and neural activity during sensory stimulation were preserved over 5 and 3 months, respectively, before optical quality deteriorated. Vascular remodeling including increased tortuosity and branching was quantified. However, capillary connectivity from arterioles to venules remained unchanged. Further, behavioral assessment before and after surgery demonstrated no impact on cognitive and motor function. Immunohistochemistry confirmed minimal astrocyte activation with no focal damage. Over 6 months, total cortical depth visualized decreased. When under anesthesia, the most prominent isoflurane-induced vasodilation occurred in capillaries and smaller arterioles. Comparison with existing method(s): These results demonstrate the capability to repeatedly observe cortical physiology in awake marmosets over months. Conclusions: This work provides a novel and insightful technique to investigate critical mechanisms in neurological disorders in awake marmosets without introducing confounds from anesthesia. Twit Text FOAVip Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets ????J Neurosci Methods http://www.kidney.de/pget.php?&tw=1&pmid=27393311 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27393311 #FOAvip English Wikipedia <ref>{{Cite pmid|27393311}}</ref> Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets #MMPMID27393311 Santisakultarm TP; Kersbergen CJ; Bandy DK; Ide DC; Choi SH; Silva AC J Neurosci Methods 2016[Sep]; 271 (ä): 55-64 PMID27393311show ga Background: Marmosets are a powerful, emerging model for human behavior and neurological disorders. However, longitudinal imaging modalities that visualize both cellular structure and function within the cortex are not available in this animal model. Hence, we implemented an approach to quantify vascular topology, hemodynamics, and neural activity in awake marmosets using two-photon microscopy (2PM). New method: Marmosets were acclimated to a custom stereotaxic system. AAV1-GCaMP5G was injected into somatosensory cortex to optically indicate neural activity, and a cranial chamber was implanted. Results: Longitudinal 2PM revealed vasculature and neurons 500 µm below the cortical surface. Vascular response and neural activity during sensory stimulation were preserved over 5 and 3 months, respectively, before optical quality deteriorated. Vascular remodeling including increased tortuosity and branching was quantified. However, capillary connectivity from arterioles to venules remained unchanged. Further, behavioral assessment before and after surgery demonstrated no impact on cognitive and motor function. Immunohistochemistry confirmed minimal astrocyte activation with no focal damage. Over 6 months, total cortical depth visualized decreased. When under anesthesia, the most prominent isoflurane-induced vasodilation occurred in capillaries and smaller arterioles. Comparison with existing method(s): These results demonstrate the capability to repeatedly observe cortical physiology in awake marmosets over months. Conclusions: This work provides a novel and insightful technique to investigate critical mechanisms in neurological disorders in awake marmosets without introducing confounds from anesthesia. äTwo-photon imaging of cerebral hemodynamics and neural activity in awa(epmc).pdf DeepDyve Pubget Overpricing