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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Neurosci+Methods
2016 ; 271
(ä): 55-64
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Two-photon imaging of cerebral hemodynamics and neural activity in awake and
anesthetized marmosets
#MMPMID27393311
Santisakultarm TP
; Kersbergen CJ
; Bandy DK
; Ide DC
; Choi SH
; Silva AC
J Neurosci Methods
2016[Sep]; 271
(ä): 55-64
PMID27393311
show ga
BACKGROUND: Marmosets are a powerful, emerging model for human behavior and
neurological disorders. However, longitudinal imaging modalities that visualize
both cellular structure and function within the cortex are not available in this
animal model. Hence, we implemented an approach to quantify vascular topology,
hemodynamics, and neural activity in awake marmosets using two-photon microscopy
(2PM). NEW METHOD: Marmosets were acclimated to a custom stereotaxic system.
AAV1-GCaMP5G was injected into somatosensory cortex to optically indicate neural
activity, and a cranial chamber was implanted. RESULTS: Longitudinal 2PM revealed
vasculature and neurons 500?m below the cortical surface. Vascular response and
neural activity during sensory stimulation were preserved over 5 and 3 months,
respectively, before optical quality deteriorated. Vascular remodeling including
increased tortuosity and branching was quantified. However, capillary
connectivity from arterioles to venules remained unchanged. Further, behavioral
assessment before and after surgery demonstrated no impact on cognitive and motor
function. Immunohistochemistry confirmed minimal astrocyte activation with no
focal damage. Over 6 months, total cortical depth visualized decreased. When
under anesthesia, the most prominent isoflurane-induced vasodilation occurred in
capillaries and smaller arterioles. COMPARISON WITH EXISTING METHOD(S): These
results demonstrate the capability to repeatedly observe cortical physiology in
awake marmosets over months. CONCLUSIONS: This work provides a novel and
insightful technique to investigate critical mechanisms in neurological disorders
in awake marmosets without introducing confounds from anesthesia.
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