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10.3390/microarrays1030107

http://scihub22266oqcxt.onion/10.3390/microarrays1030107
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C5003434!5003434!27605339
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suck abstract from ncbi


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pmid27605339      Microarrays+(Basel) 2012 ; 1 (3): 107-24
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  • Integrated Amplification Microarrays for Infectious Disease Diagnostics #MMPMID27605339
  • Chandler DP; Bryant L; Griesemer SB; Gu R; Knickerbocker C; Kukhtin A; Parker J; Zimmerman C; George KS; Cooney CG
  • Microarrays (Basel) 2012[Dec]; 1 (3): 107-24 PMID27605339show ga
  • This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.
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