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2016 ; 17
(8
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Zeb1 Is a Potential Regulator of Six2 in the Proliferation, Apoptosis and
Migration of Metanephric Mesenchyme Cells
#MMPMID27509493
Gu Y
; Zhao Y
; Zhou Y
; Xie Y
; Ju P
; Long Y
; Liu J
; Ni D
; Cao F
; Lyu Z
; Mao Z
; Hao J
; Li Y
; Wan Q
; Kanyomse Q
; Liu Y
; Ren D
; Ning Y
; Li X
; Zhou Q
; Li B
Int J Mol Sci
2016[Aug]; 17
(8
): ä PMID27509493
show ga
Nephron progenitor cells surround around the ureteric bud tips (UB) and
inductively interact with the UB to originate nephrons, the basic units of renal
function. This process is determined by the internal balance between self-renewal
and consumption of the nephron progenitor cells, which is depending on the
complicated regulation networks. It has been reported that Zeb1 regulates the
proliferation of mesenchymal cells in mouse embryos. However, the role of Zeb1 in
nephrons generation is not clear, especially in metanephric mesenchyme (MM).
Here, we detected cell proliferation, apoptosis and migration in MM cells by EdU
assay, flow cytometry assay and wound healing assay, respectively. Meanwhile,
Western and RT-PCR were used to measure the expression level of Zeb1 and Six2 in
MM cells and developing kidney. Besides, the dual-luciferase assay was conducted
to study the molecular relationship between Zeb1 and Six2. We found that
knock-down of Zeb1 decreased cell proliferation, migration and promoted cell
apoptosis in MM cells and Zeb1 overexpression leaded to the opposite data.
Western-blot and RT-PCR results showed that knock-down of Zeb1 decreased the
expression of Six2 in MM cells and Zeb1 overexpression contributed to the
opposite results. Similarly, Zeb1 promoted Six2 promoter reporter activity in
luciferase assays. However, double knock-down of Zeb1 and Six2 did not enhance
the apoptosis of MM cells compared with control cells. Nevertheless, double
silence of Zeb1 and Six2 repressed cell proliferation. In addition, we also found
that Zeb1 and Six2 had an identical pattern in distinct developing phases of
embryonic kidney. These results indicated that there may exist a complicated
regulation network between Six2 and Zeb1. Together, we demonstrate Zeb1 promotes
proliferation and apoptosis and inhibits the migration of MM cells, in
association with Six2.
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