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10.1038/srep31730 http://scihub22266oqcxt.onion/10.1038/srep31730 C4999880!4999880!27562653     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=27562653|t=2016. Complete De Novo Assembly of Monoclonal Antibody Sequences |pdf=|usr=}}{{27562653}} gab.com Text Complete De Novo Assembly of Monoclonal Antibody Sequences JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27562653 #FOAvip De novo protein sequencing is one of the key problems in mass spectrometry-based proteomics, especially for novel proteins such as monoclonal antibodies for which genome information is often limited or not available. However, due to limitations in peptides fragmentation and coverage, as well as ambiguities in spectra interpretation, complete de novo assembly of unknown protein sequences still remains challenging. To address this problem, we propose an integrated system, ALPS, which for the first time can automatically assemble full-length monoclonal antibody sequences. Our system integrates de novo sequencing peptides, their quality scores and error-correction information from databases into a weighted de Bruijn graph to assemble protein sequences. We evaluated ALPS performance on two antibody data sets, each including a heavy chain and a light chain. The results show that ALPS was able to assemble three complete monoclonal antibody sequences of length 216?441 AA, at 100% coverage, and 96.64?100% accuracy. Twit Text FOAVip Complete De Novo Assembly of Monoclonal Antibody Sequences ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27562653 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27562653 #FOAvip English Wikipedia <ref>{{Cite pmid|27562653}}</ref> Complete De Novo Assembly of Monoclonal Antibody Sequences #MMPMID27562653 Tran NH; Rahman MZ; He L; Xin L; Shan B; Li M Sci Rep 2016[]; 6 (ä): ä PMID27562653show ga De novo protein sequencing is one of the key problems in mass spectrometry-based proteomics, especially for novel proteins such as monoclonal antibodies for which genome information is often limited or not available. However, due to limitations in peptides fragmentation and coverage, as well as ambiguities in spectra interpretation, complete de novo assembly of unknown protein sequences still remains challenging. To address this problem, we propose an integrated system, ALPS, which for the first time can automatically assemble full-length monoclonal antibody sequences. Our system integrates de novo sequencing peptides, their quality scores and error-correction information from databases into a weighted de Bruijn graph to assemble protein sequences. We evaluated ALPS performance on two antibody data sets, each including a heavy chain and a light chain. The results show that ALPS was able to assemble three complete monoclonal antibody sequences of length 216?441 AA, at 100% coverage, and 96.64?100% accuracy. äComplete De Novo Assembly of Monoclonal Antibody Sequences (epmc).pdf DeepDyve Pubget Overpricing