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2016 ; 12
(3
): 1651-1656
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Identification and isolation of kidney-derived stem cells from transgenic rats
with diphtheria toxin-induced kidney damage
#MMPMID27588086
Liu QZ
; Chen XD
; Liu G
; Guan GJ
Exp Ther Med
2016[Sep]; 12
(3
): 1651-1656
PMID27588086
show ga
Adult stem cells have been well characterized in numerous organs, with the
exception of the kidneys. Therefore, the present study aimed to identify and
isolate kidney-derived stem cells. A total of 12 Fischer 344 transgenic rats
expressing the human diphtheria toxin receptor in podocyte cells of the kidney,
were used in the present study. The rats were administered
5-bromo-2'-deoxyuridine (BrdU) in order to detect cellular proliferation. After
60 days, the rats were treated with the diphtheria toxin (DT), in order to induce
kidney injury. Immunohistochemical analysis indicated that the number of
BrdU-positive cells were increased following DT treatment. In addition, the
expression of octamer-binding transcription factor 4 (Oct-4), a stem cell marker,
was detected and suggested that kidney-specific stem cells were present in the
DT-treated tissue samples. Furthermore, tissue samples exhibited repair of the
DT-induced injury. Further cellular culturing was conducted in order to isolate
the kidney-specific stem cells. After 5 weeks of culture, the majority of the
cells were non-viable, with the exception of certain specialized, unique cell
types, which were monomorphic and spindle-shaped in appearance. The unique cells
were isolated and subjected to immunostaining and reverse
transcription-polymerase chain reaction analyses in order to reconfirm the
expression of Oct-4 and to detect the expression of Paired box 2 (Pax-2), which
is necessary for the formation of kidney structures. The unique cells were
positive for Oct-4 and Pax-2; thus suggesting that the identified cells were
kidney-derived stem cells. The results of the present study suggested that the
unique cell type identified in the kidneys of the DT-treated rats were
kidney-specific stem cells that may have been involved in the repair of
DT-induced tissue injury. In addition, these cells may provide a useful cell line
for studying the fundamental characteristics of kidney stem cells, as well as
identifying kidney-specific stem cell markers.
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