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10.1038/ncomms12314 http://scihub22266oqcxt.onion/10.1038/ncomms12314 C4996941!4996941!27545823     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=27545823|t=2016. Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography |pdf=|usr=}}{{27545823}} gab.com Text Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27545823 #FOAvip Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3?Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1?ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1?mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX. Twit Text FOAVip Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27545823 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27545823 #FOAvip English Wikipedia <ref>{{Cite pmid|27545823}}</ref> Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography #MMPMID27545823 Nogly P; Panneels V; Nelson G; Gati C; Kimura T; Milne C; Milathianaki D; Kubo M; Wu W; Conrad C; Coe J; Bean R; Zhao Y; Båth P; Dods R; Harimoorthy R; Beyerlein KR; Rheinberger J; James D; DePonte D; Li C; Sala L; Williams GJ; Hunter MS; Koglin JE; Berntsen P; Nango E; Iwata S; Chapman HN; Fromme P; Frank M; Abela R; Boutet S; Barty A; White TA; Weierstall U; Spence J; Neutze R; Schertler G; Standfuss J Nat Commun 2016[]; 7 (ä): ä PMID27545823show ga Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3?Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1?ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1?mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX. äLipidic cubic phase injector is a viable crystal delivery system for t(epmc).pdf DeepDyve Pubget Overpricing