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10.1002/0471142727.mb2133s114 http://scihub22266oqcxt.onion/10.1002/0471142727.mb2133s114 C4968042!4968042!27038388     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Mol+Biol 2016 ; 114 (ä): 21.33.1-21.33.11 Nephropedia Template TP {{tp|p=27038388|t=2016. Molecular Dissection of Chromatin Maturation Via Click Chemistry |pdf=|usr=}}{{27038388}} gab.com Text Molecular Dissection of Chromatin Maturation Via Click Chemistry JO: Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27038388 #FOAvip DNA synthesis and chromatin assembly are coordinated and well regulated and comprise the two most critical processes of eukaryotic cell division, Although the interplay between DNA and its higher-order chromatin state is integral for many processes, including cell survival and genome stability, little is known about the reestablishment of chromatin structure during the cell cycle. Moreover, the extent to which the fidelity of the newly synthesized chromatin plays a role in the maintenance of cellular identity is still under debate. Here, we present a novel approach to purify nascent chromatin from the replication fork. In this protocol, we take advantage of click chemistry, a method that allows efficient conjugation of azide-containing biotin molecules to ethynyl-labeled nucleic acids. Using this approach, we selectively enrich biotin-nucleic acid conjugates via streptavidin affinity purification to pull down and assess chromatin states as well as chromatin-bound complexes from newly replicated DNA fragments with western blot and/or mass spectrometry. Twit Text FOAVip Molecular Dissection of Chromatin Maturation Via Click Chemistry ????Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27038388 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27038388 #FOAvip English Wikipedia <ref>{{Cite pmid|27038388}}</ref> Molecular Dissection of Chromatin Maturation Via Click Chemistry #MMPMID27038388 Yildirim O; Kingston RE Curr Protoc Mol Biol 2016[]; 114 (ä): 21.33.1-21.33.11 PMID27038388show ga DNA synthesis and chromatin assembly are coordinated and well regulated and comprise the two most critical processes of eukaryotic cell division, Although the interplay between DNA and its higher-order chromatin state is integral for many processes, including cell survival and genome stability, little is known about the reestablishment of chromatin structure during the cell cycle. Moreover, the extent to which the fidelity of the newly synthesized chromatin plays a role in the maintenance of cellular identity is still under debate. Here, we present a novel approach to purify nascent chromatin from the replication fork. In this protocol, we take advantage of click chemistry, a method that allows efficient conjugation of azide-containing biotin molecules to ethynyl-labeled nucleic acids. Using this approach, we selectively enrich biotin-nucleic acid conjugates via streptavidin affinity purification to pull down and assess chromatin states as well as chromatin-bound complexes from newly replicated DNA fragments with western blot and/or mass spectrometry. äMolecular Dissection of Chromatin Maturation Via Click Chemistry (epmc).pdf DeepDyve Pubget Overpricing