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10.1002/cpmb.14 http://scihub22266oqcxt.onion/10.1002/cpmb.14 C4963815!4963815!27366890     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Mol+Biol 2016 ; 115 (ä): 31.4.1-6 Nephropedia Template TP {{tp|p=27366890|t=2016. Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology |pdf=|usr=}}{{27366890}} gab.com Text Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology JO: Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27366890 #FOAvip Until very recently, the prospect of introducing mutations or exogenous DNA sequences at precise locations in the genomes of plants and animals was difficult, if not impossible. This rapidly changed with the demonstration that the type II CRISPR-Cas complex, a bacterial anti-viral surveillance system, could be engineered into a simple and robust platform for introducing double-stranded DNA breaks at nearly any position of plant and animal genomes. The prospect of efficiently creating tailored changes to a gene of interest is revolutionizing biomedical research, allowing exciting new questions to be asked. This overview introduces CRISPR-Cas technology as a tool for molecular biology and briefly discusses the advantages of this method over earlier techniques, as well as unique opportunities to create new avenues of research. Twit Text FOAVip Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology ????Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27366890 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27366890 #FOAvip English Wikipedia <ref>{{Cite pmid|27366890}}</ref> Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology #MMPMID27366890 Newman M; Ausubel FM Curr Protoc Mol Biol 2016[]; 115 (ä): 31.4.1-6 PMID27366890show ga Until very recently, the prospect of introducing mutations or exogenous DNA sequences at precise locations in the genomes of plants and animals was difficult, if not impossible. This rapidly changed with the demonstration that the type II CRISPR-Cas complex, a bacterial anti-viral surveillance system, could be engineered into a simple and robust platform for introducing double-stranded DNA breaks at nearly any position of plant and animal genomes. The prospect of efficiently creating tailored changes to a gene of interest is revolutionizing biomedical research, allowing exciting new questions to be asked. This overview introduces CRISPR-Cas technology as a tool for molecular biology and briefly discusses the advantages of this method over earlier techniques, as well as unique opportunities to create new avenues of research. äIntroduction to Gene Editing and Manipulation Using CRISPR/Cas9 Techno(epmc).pdf DeepDyve Pubget Overpricing