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10.1038/ncomms12202 http://scihub22266oqcxt.onion/10.1038/ncomms12202 C4961840!4961840!27447739     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=27447739|t=2016. Phenotypes on demand via switchable target protein degradation in multicellular organisms |pdf=|usr=}}{{27447739}} gab.com Text Phenotypes on demand via switchable target protein degradation in multicellular organisms JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27447739 #FOAvip Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversible and switch-like tuning of protein levels under physiological conditions in vivo. Thereby, developmental effects can be triggered and phenotypes on demand generated. The lt-degron was established to produce conditional and cell-type-specific phenotypes and is generally applicable in a wide range of organisms, from eukaryotic microorganisms to plants and poikilothermic animals. We have successfully applied this system to control the abundance and function of transcription factors and different enzymes by tunable protein accumulation. Twit Text FOAVip Phenotypes on demand via switchable target protein degradation in multicellular organisms ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27447739 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27447739 #FOAvip English Wikipedia <ref>{{Cite pmid|27447739}}</ref> Phenotypes on demand via switchable target protein degradation in multicellular organisms #MMPMID27447739 Faden F; Ramezani T; Mielke S; Almudi I; Nairz K; Froehlich MS; Höckendorff J; Brandt W; Hoehenwarter W; Dohmen RJ; Schnittger A; Dissmeyer N Nat Commun 2016[]; 7 (ä): ä PMID27447739show ga Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversible and switch-like tuning of protein levels under physiological conditions in vivo. Thereby, developmental effects can be triggered and phenotypes on demand generated. The lt-degron was established to produce conditional and cell-type-specific phenotypes and is generally applicable in a wide range of organisms, from eukaryotic microorganisms to plants and poikilothermic animals. We have successfully applied this system to control the abundance and function of transcription factors and different enzymes by tunable protein accumulation. äPhenotypes on demand via switchable target protein degradation in mult(epmc).pdf DeepDyve Pubget Overpricing