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10.1038/nmeth.3804 http://scihub22266oqcxt.onion/10.1038/nmeth.3804 C4941813!4941813!27018580     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2016 ; 13 (5): 439-42 Nephropedia Template TP {{tp|p=27018580|t=2016. Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis |pdf=|usr=}}{{27018580}} gab.com Text Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip Current super-resolution techniques offer unprecedented spatial resolution, but quantitative counting of spatially unresolvable molecules remains challenging. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative Points Accumulation In Nanoscale Topography (qPAINT), avoids the challenging task of analyzing the environmentally sensitive hard-to-predict photophysics of dyes, and enables robust counting by analyzing the predictable binding kinetics of dye-labeled DNA probes. We benchmarked qPAINT in vitro and in situ by counting strands on DNA nanostructures, Nup98 protein clusters in the nuclear pore complex, Bruchpilot proteins in Drosophila, and finally the number of fluorescence in situ hybridization probes on single mRNA targets in fixed cells. We achieved high accuracy (~98?99 %), high precision (~80?95 %), and multiplexed detection over a large dynamic range. Twit Text FOAVip Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27018580 #FOAvip English Wikipedia <ref>{{Cite pmid|27018580}}</ref> Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis #MMPMID27018580 Jungmann R; Avendaņo MS; Dai M; Woehrstein JB; Agasti SS; Feiger Z; Rodal A; Yin P Nat Methods 2016[May]; 13 (5): 439-42 PMID27018580show ga Current super-resolution techniques offer unprecedented spatial resolution, but quantitative counting of spatially unresolvable molecules remains challenging. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative Points Accumulation In Nanoscale Topography (qPAINT), avoids the challenging task of analyzing the environmentally sensitive hard-to-predict photophysics of dyes, and enables robust counting by analyzing the predictable binding kinetics of dye-labeled DNA probes. We benchmarked qPAINT in vitro and in situ by counting strands on DNA nanostructures, Nup98 protein clusters in the nuclear pore complex, Bruchpilot proteins in Drosophila, and finally the number of fluorescence in situ hybridization probes on single mRNA targets in fixed cells. We achieved high accuracy (~98?99 %), high precision (~80?95 %), and multiplexed detection over a large dynamic range. äQuantitative Super-Resolution Imaging with qPAINT using Transient Bind(epmc).pdf DeepDyve Pubget Overpricing