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Pseudo-ligandless Click Chemistry for Oligonucleotide Conjugation #MMPMID27258688
Mack S; Fouz MF; Dey SK; Das SR
Curr Protoc Chem Biol 2016[]; 8 (2): 83-95 PMID27258688show ga
Particularly for its use in bioconjugations, the copper-catalyzed (or promoted) azide-alkyne cycloaddition (CuAAC) reaction or ?click chemistry?, has become an essential component of the modern chemical biologist?s toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocol presented here can be used for either. The reaction can be carried out in aqueous buffer and uses acetonitrile as a minor co-solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product.