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10.1016/j.biomaterials.2016.06.025 http://scihub22266oqcxt.onion/10.1016/j.biomaterials.2016.06.025 C4939101!4939101!27344365     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomaterials 2016 ; 102 (ä): 220-30 Nephropedia Template TP {{tp|p=27344365|t=2016. Comparative Biology of Decellularized Lung Matrix: Implications of Species Mismatch in Regenerative Medicine |pdf=|usr=}}{{27344365}} gab.com Text Comparative Biology of Decellularized Lung Matrix: Implications of Species Mismatch in Regenerative Medicine JO: Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=27344365 #FOAvip Lung engineering is a promising technology, relying on re-seeding of either human or xenographic decellularized matrices with patient-derived pulmonary cells. Little is known about the species-specificity of decellularization in various models of lung regeneration, or if species dependent cell-matrix interactions exist within these systems. Therefore decellularized scaffolds were produced from rat, pig, primate and human lungs, and assessed by measuring residual DNA, mechanical properties, and key matrix proteins (collagen, elastin, glycosaminoglycans). To study intrinsic matrix biologic cues, human endothelial cells were seeded onto acellular slices and analyzed for markers of cell health and inflammation. Despite similar levels of collagen after decellularization, human and primate lungs were stiffer, contained more elastin, and retained fewer glycosaminoglycans than pig or rat lung scaffolds. Human endothelial cells seeded onto human and primate lung tissue demonstrated less expression of vascular cell adhesion molecule and activation of nuclear factor-?B compared to those seeded onto rodent or porcine tissue. Adhesion of endothelial cells was markedly enhanced on human and primate tissues. Our work suggests that species-dependent biologic cues intrinsic to lung extracellular matrix could have profound effects on attempts at lung regeneration. Twit Text FOAVip Comparative Biology of Decellularized Lung Matrix: Implications of Species Mismatch in Regenerative Medicine ????Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=27344365 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27344365 #FOAvip English Wikipedia <ref>{{Cite pmid|27344365}}</ref> Comparative Biology of Decellularized Lung Matrix: Implications of Species Mismatch in Regenerative Medicine #MMPMID27344365 Balestrini JL; Gard AL; Gerhold KA; Wilcox EC; Liu A; Schwan J; Le AV; Baevova P; Dimitrievska S; Zhao L; Sundaram S; Sun H; Rittié L; Dyal R; Broekelmann TJ; Mecham RP; Schwartz MA; Niklason LE; White ES Biomaterials 2016[Sep]; 102 (ä): 220-30 PMID27344365show ga Lung engineering is a promising technology, relying on re-seeding of either human or xenographic decellularized matrices with patient-derived pulmonary cells. Little is known about the species-specificity of decellularization in various models of lung regeneration, or if species dependent cell-matrix interactions exist within these systems. Therefore decellularized scaffolds were produced from rat, pig, primate and human lungs, and assessed by measuring residual DNA, mechanical properties, and key matrix proteins (collagen, elastin, glycosaminoglycans). To study intrinsic matrix biologic cues, human endothelial cells were seeded onto acellular slices and analyzed for markers of cell health and inflammation. Despite similar levels of collagen after decellularization, human and primate lungs were stiffer, contained more elastin, and retained fewer glycosaminoglycans than pig or rat lung scaffolds. Human endothelial cells seeded onto human and primate lung tissue demonstrated less expression of vascular cell adhesion molecule and activation of nuclear factor-?B compared to those seeded onto rodent or porcine tissue. Adhesion of endothelial cells was markedly enhanced on human and primate tissues. Our work suggests that species-dependent biologic cues intrinsic to lung extracellular matrix could have profound effects on attempts at lung regeneration. äComparative Biology of Decellularized Lung Matrix: Implications of Spe(epmc).pdf DeepDyve Pubget Overpricing