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10.1074/mcp.M115.057448 http://scihub22266oqcxt.onion/10.1074/mcp.M115.057448 C4937503!4937503 !27114453     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27114453 &cmd=llinks): Failed to open stream: HTTP request failed! 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Multidimensional Proteomics Reveals a Role of UHRF2 in the Regulation of Epithelial-Mesenchymal Transition (EMT) |pdf=|usr=}}{{27114453 }} gab.com Text Multidimensional Proteomics Reveals a Role of UHRF2 in the Regulation of Epithelial-Mesenchymal Transition (EMT) JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=27114453 #FOAvip UHRF1 is best known for its positive role in the maintenance of DNMT1-mediated DNA methylation and is implicated in a variety of tumor processes. In this paper, we provided evidence to demonstrate a role of UHRF2 in cell motility and invasion through the regulation of the epithelial-mesenchymal transition (EMT) process by acting as a transcriptional co-regulator of the EMT-transcription factors (TFs). We ectopically expressed UHRF2 in gastric cancer cell lines and performed multidimensional proteomics analyses. Proteome profiling analysis suggested a role of UHRF2 in repression of cell-cell adhesion; analysis of proteome-wide TF DNA binding activities revealed the up-regulation of many EMT-TFs in UHRF2-overexpressing cells. These data suggest that UHRF2 is a regulator of cell motility and the EMT program. Indeed, cell invasion experiments demonstrated that silencing of UHRF2 in aggressive cells impaired their abilities of migration and invasion in vitro Further ChIP-seq identified UHRF2 genomic binding motifs that coincide with several TF binding motifs including EMT-TFs, and the binding of UHRF2 to CDH1 promoter was validated by ChIP-qPCR. Moreover, the interactome analysis with IP-MS uncovered the interaction of UHRF2 with TFs including TCF7L2 and several protein complexes that regulate chromatin remodeling and histone modifications, suggesting that UHRF2 is a transcription co-regulator for TFs such as TCF7L2 to regulate the EMT process. Taken together, our study identified a role of UHRF2 in EMT and tumor metastasis and demonstrated an effective approach to obtain clues of UHRF2 function without prior knowledge through combining evidence from multidimensional proteomics analyses. Twit Text FOAVip Multidimensional Proteomics Reveals a Role of UHRF2 in the Regulation of Epithelial-Mesenchymal Transition (EMT) ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=27114453 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27114453 #FOAvip English Wikipedia <ref>{{Cite pmid|27114453 }}</ref> Multidimensional Proteomics Reveals a Role of UHRF2 in the Regulation of Epithelial-Mesenchymal Transition (EMT) #MMPMID27114453 Lai M ; Liang L ; Chen J ; Qiu N ; Ge S ; Ji S ; Shi T ; Zhen B ; Liu M ; Ding C ; Wang Y ; Qin J Mol Cell Proteomics 2016[Jul]; 15 (7 ): 2263-78 PMID27114453 show ga UHRF1 is best known for its positive role in the maintenance of DNMT1-mediated DNA methylation and is implicated in a variety of tumor processes. In this paper, we provided evidence to demonstrate a role of UHRF2 in cell motility and invasion through the regulation of the epithelial-mesenchymal transition (EMT) process by acting as a transcriptional co-regulator of the EMT-transcription factors (TFs). We ectopically expressed UHRF2 in gastric cancer cell lines and performed multidimensional proteomics analyses. Proteome profiling analysis suggested a role of UHRF2 in repression of cell-cell adhesion; analysis of proteome-wide TF DNA binding activities revealed the up-regulation of many EMT-TFs in UHRF2-overexpressing cells. These data suggest that UHRF2 is a regulator of cell motility and the EMT program. Indeed, cell invasion experiments demonstrated that silencing of UHRF2 in aggressive cells impaired their abilities of migration and invasion in vitro Further ChIP-seq identified UHRF2 genomic binding motifs that coincide with several TF binding motifs including EMT-TFs, and the binding of UHRF2 to CDH1 promoter was validated by ChIP-qPCR. Moreover, the interactome analysis with IP-MS uncovered the interaction of UHRF2 with TFs including TCF7L2 and several protein complexes that regulate chromatin remodeling and histone modifications, suggesting that UHRF2 is a transcription co-regulator for TFs such as TCF7L2 to regulate the EMT process. Taken together, our study identified a role of UHRF2 in EMT and tumor metastasis and demonstrated an effective approach to obtain clues of UHRF2 function without prior knowledge through combining evidence from multidimensional proteomics analyses. |*Epithelial-Mesenchymal Transition [MESH] |Antigens, CD [MESH] |Binding Sites [MESH] |Cadherins/genetics/metabolism [MESH] |Cell Adhesion [MESH] |Cell Line, Tumor [MESH] |Cell Movement [MESH] |Gene Expression Regulation, Neoplastic [MESH] |Humans [MESH] |Promoter Regions, Genetic [MESH] |Proteomics/*methods [MESH] |Stomach Neoplasms/*metabolism [MESH] |Transcription Factor 7-Like 2 Protein/genetics/metabolism [MESH] |Transcription Factors/genetics/*metabolism [MESH] |Ubiquitin-Protein Ligases/chemistry/*metabolism [MESH]Multidimensional Proteomics Reveals a Role of UHRF2 in the Regulation (epmc).pdf DeepDyve Pubget Overpricing