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10.1152/ajpheart.00665.2015

http://scihub22266oqcxt.onion/10.1152/ajpheart.00665.2015
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suck abstract from ncbi


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pmid27016580      Am+J+Physiol+Heart+Circ+Physiol 2016 ; 310 (11): H1388-401
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  • A technical review of optical mapping of intracellular calcium within myocardial tissue #MMPMID27016580
  • Jaimes R; Walton RD; Pasdois P; Bernus O; Efimov IR; Kay MW
  • Am J Physiol Heart Circ Physiol 2016[Jun]; 310 (11): H1388-401 PMID27016580show ga
  • Optical mapping of Ca2+-sensitive fluorescence probes has become an extremely useful approach and adopted by many cardiovascular research laboratories to study a spectrum of myocardial physiology and disease conditions. Optical mapping data are often displayed as detailed pseudocolor images, providing unique insight for interpreting mechanisms of ectopic activity, action potential and Ca2+ transient alternans, tachycardia, and fibrillation. Ca2+-sensitive fluorescent probes and optical mapping systems continue to evolve in the ongoing effort to improve therapies that ease the growing worldwide burden of cardiovascular disease. In this technical review we provide an updated overview of conventional approaches for optical mapping of Cai2+ within intact myocardium. In doing so, a brief history of Cai2+ probes is provided, and nonratiometric and ratiometric Ca2+ probes are discussed, including probes for imaging sarcoplasmic reticulum Ca2+ and probes compatible with potentiometric dyes for dual optical mapping. Typical measurements derived from optical Cai2+ signals are explained, and the analytics used to compute them are presented. Last, recent studies using Cai2+ optical mapping to study arrhythmias, heart failure, and metabolic perturbations are summarized.
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