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10.1002/jcp.24614 http://scihub22266oqcxt.onion/10.1002/jcp.24614 C4926039!4926039 !24619927     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24619927 &cmd=llinks): Failed to open stream: HTTP request failed! 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Nanotopography directs mesenchymal stem cells to osteoblast lineage through regulation of microRNA-SMAD-BMP-2 circuit |pdf=|usr=}}{{24619927 }} gab.com Text Nanotopography directs mesenchymal stem cells to osteoblast lineage through regulation of microRNA-SMAD-BMP-2 circuit JO: J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=24619927 #FOAvip The aim of this study was to investigate if chemically produced nanotopography on titanium (Ti) surface induces osteoblast differentiation of cultured human bone marrow mesenchymal stem cells (hMSCs) by regulating the expression of microRNAs (miRs). It was demonstrated that Ti with nanotopography induces osteoblast differentiation of hMSCs as evidenced by upregulation of osteoblast specific markers compared with untreated (control) Ti at day 4. At this time-point, miR-sequencing analysis revealed that 20 miRs were upregulated (>twofold) while 20 miRs were downregulated (>threefold) in hMSCs grown on Ti with nanotopography compared with control Ti. Three miRs, namely miR-4448, -4708, and -4773, which were significantly downregulated (>fivefold) by Ti with nanotopography affect osteoblast differentiation of hMSCs. These miRs directly target SMAD1 and SMAD4, both key transducers of the bone morphogenetic protein 2 (BMP-2) osteogenic signal, which were upregulated by Ti with nanotopography. Overexpression of miR-4448, -4708, and 4773 in MC3T3-E1 pre-osteoblasts noticeably inhibited gene and protein expression of SMAD1 and SMAD4 and therefore repressed the gene expression of key bone markers. Additionally, it was observed that the treatment with BMP-2 displayed a higher osteogenic effect on MC3T3-E1 cells grown on Ti with nanotopography compared with control Ti, suggesting that the BMP-2 signaling pathway was more effective on this surface. Taken together, these results indicate that a complex regulatory network involving a miR-SMAD-BMP-2 circuit governs the osteoblast differentiation induced by Ti with nanotopography. J. Cell. Physiol. 229: 1690-1696, 2014. © 2014 Wiley Periodicals, Inc. Twit Text FOAVip Nanotopography directs mesenchymal stem cells to osteoblast lineage through regulation of microRNA-SMAD-BMP-2 circuit ????J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=24619927 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24619927 #FOAvip English Wikipedia <ref>{{Cite pmid|24619927 }}</ref> Nanotopography directs mesenchymal stem cells to osteoblast lineage through regulation of microRNA-SMAD-BMP-2 circuit #MMPMID24619927 Kato RB ; Roy B ; De Oliveira FS ; Ferraz EP ; De Oliveira PT ; Kemper AG ; Hassan MQ ; Rosa AL ; Beloti MM J Cell Physiol 2014[Nov]; 229 (11 ): 1690-6 PMID24619927 show ga The aim of this study was to investigate if chemically produced nanotopography on titanium (Ti) surface induces osteoblast differentiation of cultured human bone marrow mesenchymal stem cells (hMSCs) by regulating the expression of microRNAs (miRs). It was demonstrated that Ti with nanotopography induces osteoblast differentiation of hMSCs as evidenced by upregulation of osteoblast specific markers compared with untreated (control) Ti at day 4. At this time-point, miR-sequencing analysis revealed that 20 miRs were upregulated (>twofold) while 20 miRs were downregulated (>threefold) in hMSCs grown on Ti with nanotopography compared with control Ti. Three miRs, namely miR-4448, -4708, and -4773, which were significantly downregulated (>fivefold) by Ti with nanotopography affect osteoblast differentiation of hMSCs. These miRs directly target SMAD1 and SMAD4, both key transducers of the bone morphogenetic protein 2 (BMP-2) osteogenic signal, which were upregulated by Ti with nanotopography. Overexpression of miR-4448, -4708, and 4773 in MC3T3-E1 pre-osteoblasts noticeably inhibited gene and protein expression of SMAD1 and SMAD4 and therefore repressed the gene expression of key bone markers. Additionally, it was observed that the treatment with BMP-2 displayed a higher osteogenic effect on MC3T3-E1 cells grown on Ti with nanotopography compared with control Ti, suggesting that the BMP-2 signaling pathway was more effective on this surface. Taken together, these results indicate that a complex regulatory network involving a miR-SMAD-BMP-2 circuit governs the osteoblast differentiation induced by Ti with nanotopography. J. Cell. Physiol. 229: 1690-1696, 2014. © 2014 Wiley Periodicals, Inc. |*Cell Lineage/drug effects/genetics [MESH] |Alkaline Phosphatase/genetics/metabolism [MESH] |Animals [MESH] |Biomarkers/metabolism [MESH] |Bone Morphogenetic Protein 2/*genetics/metabolism [MESH] |Cell Differentiation/drug effects/genetics [MESH] |Cell Line [MESH] |Core Binding Factor Alpha 1 Subunit/genetics/metabolism [MESH] |Female [MESH] |Humans [MESH] |Mesenchymal Stem Cells/*cytology [MESH] |Mice [MESH] |MicroRNAs/*genetics/metabolism [MESH] |Middle Aged [MESH] |Nanoparticles/*chemistry [MESH] |Osteoblasts/*cytology [MESH] |Osteocalcin/metabolism [MESH] |Osteopontin/metabolism [MESH] |Smad Proteins/*genetics [MESH] |Titanium/pharmacology [MESH]Nanotopography directs mesenchymal stem cells to osteoblast lineage th(epmc).pdf DeepDyve Pubget Overpricing