Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Identification of SLC41A3 as a novel player in magnesium homeostasis #MMPMID27349617
de Baaij JH; Arjona FJ; van den Brand M; Lavrijsen M; Lameris AL; Bindels RJ; Hoenderop JG
Sci Rep 2016[]; 6 (ä): ä PMID27349617show ga
Regulation of the body Mg2+ balance takes place in the distal convoluted tubule (DCT), where transcellular reabsorption determines the final urinary Mg2+ excretion. The basolateral Mg2+ extrusion mechanism in the DCT is still unknown, but recent findings suggest that SLC41 proteins contribute to Mg2+ extrusion. The aim of this study was, therefore, to characterize the functional role of SLC41A3 in Mg2+ homeostasis using the Slc41a3 knockout (Slc41a3?/?) mouse. By quantitative PCR analysis it was shown that Slc41a3 is the only SLC41 isoform with enriched expression in the DCT. Interestingly, serum and urine electrolyte determinations demonstrated that Slc41a3?/? mice suffer from hypomagnesemia. The intestinal Mg2+ absorption capacity was measured using the stable 25Mg2+ isotope in mice fed a low Mg2+ diet. 25Mg2+ uptake was similar in wildtype (Slc41a3+/+) and Slc41a3?/? mice, although Slc41a3?/? animals exhibited increased intestinal mRNA expression of Mg2+ transporters Trpm6 and Slc41a1. Remarkably, some of the Slc41a3?/? mice developed severe unilateral hydronephrosis. In conclusion, SLC41A3 was established as a new factor for Mg2+ handling.