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Cas-Database: web-based genome-wide guide RNA library design for gene knockout
screens using CRISPR-Cas9
#MMPMID27153724
Park J
; Kim JS
; Bae S
Bioinformatics
2016[Jul]; 32
(13
): 2017-23
PMID27153724
show ga
MOTIVATION: CRISPR-derived RNA guided endonucleases (RGENs) have been widely used
for both gene knockout and knock-in at the level of single or multiple genes.
RGENs are now available for forward genetic screens at genome scale, but single
guide RNA (sgRNA) selection at this scale is difficult. RESULTS: We develop an
online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9
nucleases from Streptococcus pyogenes (SpCas9). With an easy-to-use web
interface, Cas-Database allows users to select optimal target sequences simply by
changing the filtering conditions. Furthermore, it provides a powerful way to
select multiple optimal target sequences from thousands of genes at once for the
creation of a genome-wide library. Cas-Database also provides a web application
programming interface (web API) for advanced bioinformatics users. AVAILABILITY
AND IMPLEMENTATION: Free access at http://www.rgenome.net/cas-database/ CONTACT:
sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr SUPPLEMENTARY INFORMATION:
Supplementary data are available at Bioinformatics online.
free
free
free
