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10.1016/j.dnarep.2016.05.029 http://scihub22266oqcxt.onion/10.1016/j.dnarep.2016.05.029 C4917410!4917410!27258794     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       DNA+Repair+(Amst) 2016 ; 43 (ä): 48-56 Nephropedia Template TP {{tp|p=27258794|t=2016. Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification |pdf=|usr=}}{{27258794}} gab.com Text Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification JO: DNA Repair (Amst) http://www.kidney.de/pget.php?&tw=1&pmid=27258794 #FOAvip Activation-induced deoxycytidine deaminase (AID) initiates somatic hypermutation (SHM) and class-switch recombination (CSR) by deaminating C?U during transcription of Ig-variable (V) and Ig-switch (S) region DNA, which is essential to produce high-affinity antibodies. Here we report the crystal structure of a soluble human AID variant at 2.8 Å resolution that favors targeting WRC motifs (W = A/T, R = A/G) in vitro, and executes Ig V SHM in Ramos B-cells. A specificity loop extending away from the active site to accommodate two purine bases next to C, differs significantly in sequence, length, and conformation from APOBEC proteins Apo3A and Apo3G, which strongly favor pyrimidines at -1 and -2 positions. Individual amino acid contributions to specificity and processivity were measured in relation to a proposed ssDNA binding cleft. This study provides a structural basis for residue contributions to DNA scanning properties unique to AID, and for disease mutations in human HIGM-2 syndrome. Twit Text FOAVip Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification ????DNA Repair (Amst) http://www.kidney.de/pget.php?&tw=1&pmid=27258794 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27258794 #FOAvip English Wikipedia <ref>{{Cite pmid|27258794}}</ref> Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification #MMPMID27258794 Pham P; Afif SA; Shimoda M; Maeda K; Sakaguchi N; Pedersen LC; Goodman MF DNA Repair (Amst) 2016[Jul]; 43 (ä): 48-56 PMID27258794show ga Activation-induced deoxycytidine deaminase (AID) initiates somatic hypermutation (SHM) and class-switch recombination (CSR) by deaminating C?U during transcription of Ig-variable (V) and Ig-switch (S) region DNA, which is essential to produce high-affinity antibodies. Here we report the crystal structure of a soluble human AID variant at 2.8 Å resolution that favors targeting WRC motifs (W = A/T, R = A/G) in vitro, and executes Ig V SHM in Ramos B-cells. A specificity loop extending away from the active site to accommodate two purine bases next to C, differs significantly in sequence, length, and conformation from APOBEC proteins Apo3A and Apo3G, which strongly favor pyrimidines at -1 and -2 positions. Individual amino acid contributions to specificity and processivity were measured in relation to a proposed ssDNA binding cleft. This study provides a structural basis for residue contributions to DNA scanning properties unique to AID, and for disease mutations in human HIGM-2 syndrome. äStructural analysis of the activation-induced deoxycytidine deaminase (epmc).pdf DeepDyve Pubget Overpricing