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10.1038/srep28423 http://scihub22266oqcxt.onion/10.1038/srep28423 C4916512!4916512 !27328819     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27328819 &cmd=llinks): Failed to open stream: HTTP request failed! 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Trehalose, sucrose and raffinose are novel activators of autophagy in human keratinocytes through an mTOR-independent pathway |pdf=|usr=}}{{27328819 }} gab.com Text Trehalose, sucrose and raffinose are novel activators of autophagy in human keratinocytes through an mTOR-independent pathway JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27328819 #FOAvip Trehalose is a natural disaccharide that is found in a diverse range of organisms but not in mammals. Autophagy is a process which mediates the sequestration, lysosomal delivery and degradation of proteins and organelles. Studies have shown that trehalose exerts beneficial effects through inducing autophagy in mammalian cells. However, whether trehalose or other saccharides can activate autophagy in keratinocytes is unknown. Here, we found that trehalose treatment increased the LC3-I to LC3-II conversion, acridine orange-stained vacuoles and GFP-LC3B (LC3B protein tagged with green fluorescent protein) puncta in the HaCaT human keratinocyte cell line, indicating autophagy induction. Trehalose-induced autophagy was also observed in primary keratinocytes and the A431 epidermal cancer cell line. mTOR signalling was not affected by trehalose treatment, suggesting that trehalose induced autophagy through an mTOR-independent pathway. mTOR-independent autophagy induction was also observed in HaCaT and HeLa cells treated with sucrose or raffinose but not in glucose, maltose or sorbitol treated HaCaT cells, indicating that autophagy induction was not a general property of saccharides. Finally, although trehalose treatment had an inhibitory effect on cell proliferation, it had a cytoprotective effect on cells exposed to UVB radiation. Our study provides new insight into the saccharide-mediated regulation of autophagy in keratinocytes. Twit Text FOAVip Trehalose, sucrose and raffinose are novel activators of autophagy in human keratinocytes through an mTOR-independent pathway ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27328819 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27328819 #FOAvip English Wikipedia <ref>{{Cite pmid|27328819 }}</ref> Trehalose, sucrose and raffinose are novel activators of autophagy in human keratinocytes through an mTOR-independent pathway #MMPMID27328819 Chen X ; Li M ; Li L ; Xu S ; Huang D ; Ju M ; Huang J ; Chen K ; Gu H Sci Rep 2016[Jun]; 6 (ä): 28423 PMID27328819 show ga Trehalose is a natural disaccharide that is found in a diverse range of organisms but not in mammals. Autophagy is a process which mediates the sequestration, lysosomal delivery and degradation of proteins and organelles. Studies have shown that trehalose exerts beneficial effects through inducing autophagy in mammalian cells. However, whether trehalose or other saccharides can activate autophagy in keratinocytes is unknown. Here, we found that trehalose treatment increased the LC3-I to LC3-II conversion, acridine orange-stained vacuoles and GFP-LC3B (LC3B protein tagged with green fluorescent protein) puncta in the HaCaT human keratinocyte cell line, indicating autophagy induction. Trehalose-induced autophagy was also observed in primary keratinocytes and the A431 epidermal cancer cell line. mTOR signalling was not affected by trehalose treatment, suggesting that trehalose induced autophagy through an mTOR-independent pathway. mTOR-independent autophagy induction was also observed in HaCaT and HeLa cells treated with sucrose or raffinose but not in glucose, maltose or sorbitol treated HaCaT cells, indicating that autophagy induction was not a general property of saccharides. Finally, although trehalose treatment had an inhibitory effect on cell proliferation, it had a cytoprotective effect on cells exposed to UVB radiation. Our study provides new insight into the saccharide-mediated regulation of autophagy in keratinocytes. |Autophagy [MESH] |Cell Proliferation/drug effects [MESH] |Cells, Cultured [MESH] |HeLa Cells [MESH] |Humans [MESH] |Keratinocytes/*cytology/drug effects/metabolism [MESH] |Microtubule-Associated Proteins/metabolism [MESH] |Raffinose/*pharmacokinetics [MESH] |Signal Transduction/drug effects [MESH] |Sucrose/*pharmacology [MESH] |TOR Serine-Threonine Kinases/*metabolism [MESH]Trehalose, sucrose and raffinose are novel activators of autophagy in (epmc).pdf DeepDyve Pubget Overpricing