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10.5483/BMBRep.2016.49.4.055

http://scihub22266oqcxt.onion/10.5483/BMBRep.2016.49.4.055
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C4915236!4915236!26996342
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suck abstract from ncbi


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pmid26996342      BMB+Rep 2016 ; 49 (4): 197-8
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  • An efficient SCNT technology for the establishment of personalized and public human pluripotent stem cell banks #MMPMID26996342
  • Lee JE; Chung YG; Eum JH; Lee Y; Lee DR
  • BMB Rep 2016[Apr]; 49 (4): 197-8 PMID26996342show ga
  • Although three different research groups have reported successful derivations of human somatic cell nuclear transfer-derived embryonic stem cell (SCNT-ESC) lines using fetal, neonatal and adult fibroblasts, the extremely poor development of cloned embryos has hindered its potential applications in regenerative medicine. Recently, however, our group discovered that the severe methylation of lysine 9 in Histone H3 in a human somatic cell genome was a major SCNT reprogramming barrier, and the overexpression of KDM4A, a H3K9me3 demethylase, significantly improved the blastocyst formation of SCNT embryos. In particular, by applying this new approach, we were able to produce multiple SCNT-ES cell lines using oocytes obtained from donors whose eggs previously failed to develop to the blastocyst stage. Moreover, the success rate was closer to 25%, which is comparable to that of IVF embryos, so that our new human SCNT method seems to be a practical approach to establishing a pluripotent stem cell bank for the general public as well as for individual patients. [BMB Reports 2016; 49(4): 197-198]
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