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An efficient SCNT technology for the establishment of personalized and public
human pluripotent stem cell banks
#MMPMID26996342
Lee JE
; Chung YG
; Eum JH
; Lee Y
; Lee DR
BMB Rep
2016[Apr]; 49
(4
): 197-8
PMID26996342
show ga
Although three different research groups have reported successful derivations of
human somatic cell nuclear transfer-derived embryonic stem cell (SCNT-ESC) lines
using fetal, neonatal and adult fibroblasts, the extremely poor development of
cloned embryos has hindered its potential applications in regenerative medicine.
Recently, however, our group discovered that the severe methylation of lysine 9
in Histone H3 in a human somatic cell genome was a major SCNT reprogramming
barrier, and the overexpression of KDM4A, a H3K9me3 demethylase, significantly
improved the blastocyst formation of SCNT embryos. In particular, by applying
this new approach, we were able to produce multiple SCNT-ES cell lines using
oocytes obtained from donors whose eggs previously failed to develop to the
blastocyst stage. Moreover, the success rate was closer to 25%, which is
comparable to that of IVF embryos, so that our new human SCNT method seems to be
a practical approach to establishing a pluripotent stem cell bank for the general
public as well as for individual patients. [BMB Reports 2016; 49(4): 197-198].
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