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10.1016/j.celrep.2016.05.020 http://scihub22266oqcxt.onion/10.1016/j.celrep.2016.05.020 C4914699!4914699!27264181     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2016 ; 15 (11): 2475-87 Nephropedia Template TP {{tp|p=27264181|t=2016. Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination |pdf=|usr=}}{{27264181}} gab.com Text Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=27264181 #FOAvip Variable (V), diversity (D), and joining (J) (V(D)J) recombination is the first determinant of antigen receptor diversity. Understanding how recombination is regulated requires a comprehensive, unbiased readout of V gene usage. We have developed VDJ sequencing (VDJ-seq), a DNA-based next-generation-sequencing technique that quantitatively profiles recombination products. We reveal a 200-fold range of recombination efficiency among recombining V genes in the primary mouse Igh repertoire. We used machine learning to integrate these data with local chromatin profiles to identify combinatorial patterns of epigenetic features that associate with active VH gene recombination. These features localize downstream of VH genes and are excised by recombination, revealing a class of cis-regulatory element that governs recombination, distinct from expression. We detect two mutually exclusive chromatin signatures at these elements, characterized by CTCF/RAD21 and PAX5/IRF4, which segregate with the evolutionary history of associated VH genes. Thus, local chromatin signatures downstream of VH genes provide an essential layer of regulation that determines recombination efficiency. Twit Text FOAVip Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=27264181 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27264181 #FOAvip English Wikipedia <ref>{{Cite pmid|27264181}}</ref> Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination #MMPMID27264181 Bolland D; Koohy H; Wood A; Matheson L; Krueger F; Stubbington M; Baizan-Edge A; Chovanec P; Stubbs B; Tabbada K; Andrews S; Spivakov M; Corcoran A Cell Rep 2016[Jun]; 15 (11): 2475-87 PMID27264181show ga Variable (V), diversity (D), and joining (J) (V(D)J) recombination is the first determinant of antigen receptor diversity. Understanding how recombination is regulated requires a comprehensive, unbiased readout of V gene usage. We have developed VDJ sequencing (VDJ-seq), a DNA-based next-generation-sequencing technique that quantitatively profiles recombination products. We reveal a 200-fold range of recombination efficiency among recombining V genes in the primary mouse Igh repertoire. We used machine learning to integrate these data with local chromatin profiles to identify combinatorial patterns of epigenetic features that associate with active VH gene recombination. These features localize downstream of VH genes and are excised by recombination, revealing a class of cis-regulatory element that governs recombination, distinct from expression. We detect two mutually exclusive chromatin signatures at these elements, characterized by CTCF/RAD21 and PAX5/IRF4, which segregate with the evolutionary history of associated VH genes. Thus, local chromatin signatures downstream of VH genes provide an essential layer of regulation that determines recombination efficiency. äTwo Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Re(epmc).pdf DeepDyve Pubget Overpricing