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10.1093/nar/gkw405 http://scihub22266oqcxt.onion/10.1093/nar/gkw405 C4914124!4914124!27166376     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27166376&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2016 ; 44 (11): 5356-64 Nephropedia Template TP {{tp|p=27166376|t=2016. Cre-dependent DNA recombination activates a STING-dependent innate immune response |pdf=|usr=}}{{27166376}} gab.com Text Cre-dependent DNA recombination activates a STING-dependent innate immune response JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27166376 #FOAvip Gene-recombinase technologies, such as Cre/loxP-mediated DNA recombination, are important tools in the study of gene function, but have potential side effects due to damaging activity on DNA. Here we show that DNA recombination by Cre instigates a robust antiviral response in mammalian cells, independent of legitimate loxP recombination. This is due to the recruitment of the cytosolic DNA sensor STING, concurrent with Cre-dependent DNA damage and the accumulation of cytoplasmic DNA. Importantly, we establish a direct interplay between this antiviral response and cell?cell interactions, indicating that low cell densities in vitro could be useful to help mitigate these effects of Cre. Taking into account the wide range of interferon stimulated genes that may be induced by the STING pathway, these results have broad implications in fields such as immunology, cancer biology, metabolism and stem cell research. Further, this study sets a precedent in the field of gene-engineering, possibly applicable to other enzymatic-based genome editing technologies. Twit Text FOAVip Cre-dependent DNA recombination activates a STING-dependent innate immune response ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=27166376 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27166376 #FOAvip English Wikipedia <ref>{{Cite pmid|27166376}}</ref> Cre-dependent DNA recombination activates a STING-dependent innate immune response #MMPMID27166376 Pépin G; Ferrand J; Höning K; Jayasekara W; Cain JE; Behlke MA; Gough DJ; G. Williams BR; Hornung V; Gantier MP Nucleic Acids Res 2016[Jun]; 44 (11): 5356-64 PMID27166376show ga Gene-recombinase technologies, such as Cre/loxP-mediated DNA recombination, are important tools in the study of gene function, but have potential side effects due to damaging activity on DNA. Here we show that DNA recombination by Cre instigates a robust antiviral response in mammalian cells, independent of legitimate loxP recombination. This is due to the recruitment of the cytosolic DNA sensor STING, concurrent with Cre-dependent DNA damage and the accumulation of cytoplasmic DNA. Importantly, we establish a direct interplay between this antiviral response and cell?cell interactions, indicating that low cell densities in vitro could be useful to help mitigate these effects of Cre. Taking into account the wide range of interferon stimulated genes that may be induced by the STING pathway, these results have broad implications in fields such as immunology, cancer biology, metabolism and stem cell research. Further, this study sets a precedent in the field of gene-engineering, possibly applicable to other enzymatic-based genome editing technologies. äCre-dependent DNA recombination activates a STING-dependent innate imm(epmc).pdf DeepDyve Pubget Overpricing