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10.1016/j.cels.2016.03.010 http://scihub22266oqcxt.onion/10.1016/j.cels.2016.03.010 C4909256!4909256!27135540     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Syst 2016 ; 2 (4): 283-8 Nephropedia Template TP {{tp|p=27135540|t=2016. Optogenetic Control of Calcium Oscillation Waveform Defines NFAT as an Integrator of Calcium Load |pdf=|usr=}}{{27135540}} gab.com Text Optogenetic Control of Calcium Oscillation Waveform Defines NFAT as an Integrator of Calcium Load JO: Cell Syst http://www.kidney.de/pget.php?&tw=1&pmid=27135540 #FOAvip It is known that the calcium-dependent transcription factor NFAT initiates transcription in response to pulsatile loads of calcium signal. However, the relative contributions of calcium oscillation frequency, amplitude, and duty cycle to transcriptional activity remain unclear. Here, we engineer HeLa cells to permit optogenetic control of intracellular calcium concentration using programmable LED arrays. This approach allows us to generate calcium oscillations of constant peak amplitude, in which frequency is varied while holding duty cycle constant, or vice versa. Using this setup and mathematical modeling, we show that NFAT transcriptional activity depends more on duty cycle, defined as the proportion of the integrated calcium concentration over the oscillation period, than on frequency alone. This demonstrates that NFAT acts primarily as a signal integrator of cumulative load rather than a frequency-selective decoder. This approach resolves a fundamental question in calcium encoding and demonstrates the value of optogenetics for isolating individual dynamical components of larger signaling behaviors. Twit Text FOAVip Optogenetic Control of Calcium Oscillation Waveform Defines NFAT as an Integrator of Calcium Load ????Cell Syst http://www.kidney.de/pget.php?&tw=1&pmid=27135540 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27135540 #FOAvip English Wikipedia <ref>{{Cite pmid|27135540}}</ref> Optogenetic Control of Calcium Oscillation Waveform Defines NFAT as an Integrator of Calcium Load #MMPMID27135540 Hannanta-anan P; Chow BY Cell Syst 2016[Apr]; 2 (4): 283-8 PMID27135540show ga It is known that the calcium-dependent transcription factor NFAT initiates transcription in response to pulsatile loads of calcium signal. However, the relative contributions of calcium oscillation frequency, amplitude, and duty cycle to transcriptional activity remain unclear. Here, we engineer HeLa cells to permit optogenetic control of intracellular calcium concentration using programmable LED arrays. This approach allows us to generate calcium oscillations of constant peak amplitude, in which frequency is varied while holding duty cycle constant, or vice versa. Using this setup and mathematical modeling, we show that NFAT transcriptional activity depends more on duty cycle, defined as the proportion of the integrated calcium concentration over the oscillation period, than on frequency alone. This demonstrates that NFAT acts primarily as a signal integrator of cumulative load rather than a frequency-selective decoder. This approach resolves a fundamental question in calcium encoding and demonstrates the value of optogenetics for isolating individual dynamical components of larger signaling behaviors. äOptogenetic Control of Calcium Oscillation Waveform Defines NFAT as an(epmc).pdf DeepDyve Pubget Overpricing