Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/ncomms11786 http://scihub22266oqcxt.onion/10.1038/ncomms11786 C4906395!4906395 !27283361     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27283361 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2016 ; 7 (ä): 11786 Nephropedia Template TP {{tp|p=27283361 |t=2016. Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens |pdf=|usr=}}{{27283361 }} gab.com Text Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27283361 #FOAvip The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we compare the efficacy of genome-wide CRISPR/Cas9-mediated forward genetic screens versus gene-trap mutagenesis screens in haploid human cells, which represent the existing 'gold standard' method. This head-to-head comparison aimed to identify genes required for the endoplasmic reticulum-associated degradation (ERAD) of MHC class I molecules. The two approaches show high concordance (>70%), successfully identifying the majority of the known components of the canonical glycoprotein ERAD pathway. Both screens also identify a role for the uncharacterized gene TXNDC11, which we show encodes an EDEM2/3-associated disulphide reductase. Genome-wide CRISPR/Cas9-mediated screens together with haploid genetic screens provide a powerful addition to the forward genetic toolbox. Twit Text FOAVip Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27283361 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27283361 #FOAvip English Wikipedia <ref>{{Cite pmid|27283361 }}</ref> Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens #MMPMID27283361 Timms RT ; Menzies SA ; Tchasovnikarova IA ; Christensen LC ; Williamson JC ; Antrobus R ; Dougan G ; Ellgaard L ; Lehner PJ Nat Commun 2016[Jun]; 7 (ä): 11786 PMID27283361 show ga The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we compare the efficacy of genome-wide CRISPR/Cas9-mediated forward genetic screens versus gene-trap mutagenesis screens in haploid human cells, which represent the existing 'gold standard' method. This head-to-head comparison aimed to identify genes required for the endoplasmic reticulum-associated degradation (ERAD) of MHC class I molecules. The two approaches show high concordance (>70%), successfully identifying the majority of the known components of the canonical glycoprotein ERAD pathway. Both screens also identify a role for the uncharacterized gene TXNDC11, which we show encodes an EDEM2/3-associated disulphide reductase. Genome-wide CRISPR/Cas9-mediated screens together with haploid genetic screens provide a powerful addition to the forward genetic toolbox. |*Genetic Testing [MESH] |*Haploidy [MESH] |Animals [MESH] |CRISPR-Cas Systems/*genetics [MESH] |Endoplasmic Reticulum-Associated Degradation/*genetics [MESH] |Fluorescent Dyes/metabolism [MESH] |Genes, Reporter [MESH] |Glycoproteins/metabolism [MESH] |HEK293 Cells [MESH] |HeLa Cells [MESH] |Histocompatibility Antigens Class I/metabolism [MESH] |Humans [MESH] |Mammals/*genetics [MESH] |Oxidation-Reduction [MESH] |Protein Binding [MESH] |Protein Domains [MESH] |Thioredoxins/chemistry/metabolism [MESH]Genetic dissection of mammalian ERAD through comparative haploid and C(epmc).pdf DeepDyve Pubget Overpricing