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Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell 2016 ; 165 (4): 990-1001 Nephropedia Template TP
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Real-time imaging of translation on single mRNA transcripts in live cells #MMPMID27153499
Wang C; Han B; Zhou R; Zhuang X
Cell 2016[May]; 165 (4): 990-1001 PMID27153499show ga
Translation is under tight spatial and temporal controls to ensure protein production in the right time and place in cells. Methods that allow real-time, high-resolution visualization of translation in live cells are essential for understanding the spatiotemporal dynamics of translation regulation. Based on multivalent fluorescence amplification of the nascent polypeptide signal, we develop a method to image translation on individual mRNA molecules in real time in live cells, allowing direct visualization of translation events at the translation sites. Using this approach, we monitor transient changes of translation dynamics in responses to environmental stresses, capture distinct mobilities of individual polysomes in different subcellular compartments, and detect 3?UTR-dependent local translation and active transport of polysomes in dendrites of primary neurons.